Overview

  • Product name
  • Description
    Rabbit polyclonal to SNIP1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human SNIP1 aa 225-275 (N terminal).

  • Positive control
    • HeLa Cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab19611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at a concentration of 1 - 4 µg / mg of lysate.
    WB: 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Down-regulates NF-kappa-B signaling by competing with RELA for CREBBP/EP300 binding. Involved in the microRNA (miRNA) biogenesis.
    • Tissue specificity
      Ubiquitous, with highest expression in heart and skeletal muscle.
    • Sequence similarities
      Contains 1 FHA domain.
    • Post-translational
      modifications
      Degraded by the proteasome upon binding to the SMAD1/OAZ1/PSMB4 complex.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FHA domain containing protein SNIP1 antibody
      • FHA domain-containing protein SNIP1 antibody
      • FLJ12553 antibody
      • PMRED antibody
      • Smad nuclear interacting protein (Smad nuclear interacting) antibody
      • smad nuclear interacting protein 1 antibody
      • Smad nuclear interacting protein antibody
      • Smad nuclear-interacting protein 1 antibody
      • SNIP1 (Smad nuclear interacting protein) antibody
      • SNIP1 antibody
      • SNIP1_HUMAN antibody
      • Splicing factor arginine/serine rich 4 (Pre mRNA splicing factor SRP75) antibody
      see all

    Images



    • Predicted band size : 46 kDa


      ab19611 at 0.33µg/ml for Western blot and 1µg/mg lysate for Immunoprecipitation, staining human SNIP1 in HeLa cells (50µg WB; 1mg for IP). Detection was by chemiluminescence with an exposure time of 30 seconds.

    References

    This product has been referenced in:
    • Heyd F & Lynch KW Phosphorylation-dependent regulation of PSF by GSK3 controls CD45 alternative splicing. Mol Cell 40:126-37 (2010). WB ; Human . Read more (PubMed: 20932480) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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