Application notesPeptide ELISA: antibody detection limit dilution 1/16000.
WB: preliminary experiments give no signal but low background in human brain and testis lysates at up to 1µg/ml (note whole-cell RIPA lysates not nuclear lysates).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay be required for DNA interstrand cross-link repair. Also required for checkpoint mediated cell cycle arrest in early prophase in response to mitotic spindle poisons.
Tissue specificityExpressed in brain, heart, kidney, liver, pancreas, placenta and skeletal muscle.
Sequence similaritiesBelongs to the DNA repair metallo-beta-lactamase (DRMBL) family.
Cellular localizationNucleus. In some cells it may be found in typically 1 or 2 discrete nuclear aggregates of unknown function which also contain TP53BP1. Also found in multiple discrete nuclear foci which increase in number following treatment with ionizing radiation or interstrand cross-linking agents. These foci overlap with those formed by the MRN complex (composed of MRE11A, RAD50 and NBN) and BRCA1.