Overview

  • Product nameAnti-SNRNP200 antibody
    See all SNRNP200 primary antibodies
  • Description
    Rabbit polyclonal to SNRNP200
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 212-261 (GDEDVYGEVR EEASDDDMEG DEAVVRCTLS ANLVASGELM SSKKKDLHPR) of Human SNRNP200 (NP_054733, O75643).

  • Positive control
    • COLO205 lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferConstituents: 97% PBS, 2% Sucrose
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 244 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionPutative RNA helicase involved in the second step of RNA splicing. May promote one or more conformational changes in the dynamic network of RNA-RNA interactions in the spliceosome. Appears to catalyze an ATP-dependent unwinding of U4/U6 RNA duplices.
  • Tissue specificityWidely expressed.
  • Involvement in diseaseDefects in SNRNP200 are the cause of retinitis pigmentosa type 33 (RP33) [MIM:610359]. It is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
  • Sequence similaritiesBelongs to the helicase family. SKI2 subfamily.
    Contains 2 helicase ATP-binding domains.
    Contains 2 helicase C-terminal domains.
    Contains 2 SEC63 domains.
  • DomainComposed of two similar domains.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating signal cointegrator 1 complex subunit 3-like 1 antibody
    • ASCC3L1 antibody
    • BRR2 antibody
    • BRR2 homolog antibody
    • HELIC2 antibody
    • RP33 antibody
    • SNRNP200 antibody
    • U5 small nuclear ribonucleoprotein 200 kDa helicase antibody
    • U5 snRNP-specific 200 kDa protein antibody
    • U5-200KD antibody
    • U520_HUMAN antibody
    see all

Anti-SNRNP200 antibody images

  • Anti-SNRNP200 antibody (ab118713) at 1 µg/ml + COLO205 lysate at 10 µg

    Secondary
    Goat anti-Rabbit IgG-HRP

    Predicted band size : 244 kDa

References for Anti-SNRNP200 antibody (ab118713)

This product has been referenced in:
  • Liu YC  et al. Cytoplasmic Viral RNA-Dependent RNA Polymerase Disrupts the Intracellular Splicing Machinery by Entering the Nucleus and Interfering with Prp8. PLoS Pathog 10:e1004199 (2014). WB ; Human . Read more (PubMed: 24968230) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa S3)
Specification HeLa S3
Fixative Formaldehyde
Permeabilization Yes - Triton 0.3%
Blocking step Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 05 2012

Thank you for your reply. ICC/IF has not been tested, thus, you are eligible to use our testing discount program.

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab...

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Thank you for contacting us.

Yes, you can definitely use our testing discount program to try the antibody in IF.

Could you please let me know what type of IF you would like to use it in: ICC/IF (with cultured cells) or IHC-P (paraf...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"