• Product nameAnti-SNRP70 antibody
    See all SNRP70 primary antibodies
  • Description
    Rabbit polyclonal to SNRP70
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: RERERKEELR GGGGDMAEPS EAGDAPPDDG PPGELGPDGP DGPEEKGRDR, corresponding to amino acids 301-350 of Human SNRP70

  • Positive control
    • HepG2 cell lysate



Our Abpromise guarantee covers the use of ab51266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • RelevanceSNRP70 is an RNA-binding protein that is a specific component of the U1 small nuclear ribonucleoprotein complex and constitutes the major anti-(U1) RNP autoimmune antigen. SNRP70 contains 1 RRM (RNA recognition motif) domain and mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • RNPU1Z antibody
    • Rnulp70 antibody
    • RPU1 antibody
    • Small nuclear ribonucleoprotein 70 (U1) antibody
    • Small nuclear ribonucleoprotein 70kD polypeptide (RNP antigen) antibody
    • Small nuclear ribonucleoprotein 70kDa (U1) antibody
    • Small nuclear ribonucleoprotein 70kDa polypeptide (RNP antigen) antibody
    • snRNP70 antibody
    • Snrp 70 antibody
    • SNRP70 antibody
    • U1 70K antibody
    • U1 small nuclear ribonucleoprotein 70 kDa antibody
    • U1 small nuclear ribonucleoprotein polypeptide A antibody
    • U1 snRNP 70 kDa antibody
    • U170K antibody
    • U1AP antibody
    • U1AP1 antibody
    • U1RNP antibody
    see all

Anti-SNRP70 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling SNRP70 with ab51266 at 1/100. A Cy3 conjugated donkey anti-rabbit IgG (1/200) was used as teh secondary antibody. Positive staining shown in the nuclei of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - SNRP70. Right - Merge.
  • Anti-SNRP70 antibody (ab51266) at 0.25 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 48,62 kDa (why is the actual band size different from the predicted?)
  • ab51266 (2µg/ml) staining SNRP70 in human liver using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear stain.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab51266 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-SNRP70 antibody (ab51266)

This product has been referenced in:
  • Luo X  et al. Dynamic reorganization of the AC16 cardiomyocyte transcriptome in response to TNFa signaling revealed by integrated genomic analyses. BMC Genomics 15:155 (2014). WB . Read more (PubMed: 24564208) »
  • Franklin S  et al. Quantitative analysis of the chromatin proteome in disease reveals remodeling principles and identifies high mobility group protein B2 as a regulator of hypertrophic growth. Mol Cell Proteomics 11:M111.014258 (2012). WB . Read more (PubMed: 22270000) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 15 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-12% Bis-Tris Gel, MES running buffer)
Sample Mouse Tissue lysate - whole (Mouse whole brain tissue lysate, striatum, cortex,)
Specification Mouse whole brain tissue lysate, striatum, cortex,
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (NSC34)
Loading amount 30 µg
Specification NSC34
Gel Running Conditions Reduced Denaturing (4-12% Nupage gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 10 2011