- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-SNRP70 antibody
See all SNRP70 primary antibodies -
DescriptionRabbit polyclonal to SNRP70
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Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Cat, Dog
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Immunogen
A region within synthetic peptide: RERERKEELR GGGGDMAEPS EAGDAPPDDG PPGELGPDGP DGPEEKGRDR, corresponding to amino acids 301-350 of Human SNRP70
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Positive control
- HepG2 cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab51266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-P | Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.25 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
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RelevanceSNRP70 is an RNA-binding protein that is a specific component of the U1 small nuclear ribonucleoprotein complex and constitutes the major anti-(U1) RNP autoimmune antigen. SNRP70 contains 1 RRM (RNA recognition motif) domain and mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 6625 Human
- Entrez Gene: 20637 Mouse
- Entrez Gene: 361574 Rat
- Omim: 180740 Human
- SwissProt: Q1RMR2 Cow
- SwissProt: P08621 Human
- SwissProt: Q62376 Mouse
- Unigene: 467097 Human
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Alternative names
- RNPU1Z antibody
- Rnulp70 antibody
- RPU1 antibody
see all
Anti-SNRP70 antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRP70 antibody (ab51266)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling SNRP70 with ab51266 at 1/100. A Cy3 conjugated donkey anti-rabbit IgG (1/200) was used as teh secondary antibody. Positive staining shown in the nuclei of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - SNRP70. Right - Merge. -
Western blot - SNRP70 antibody (ab51266)Anti-SNRP70 antibody (ab51266) at 0.25 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 52 kDa
Observed band size : 48,62 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRP70 antibody (ab51266)ab51266 (2µg/ml) staining SNRP70 in human liver using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear stain.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-SNRP70 antibody (ab51266)ICC/IF image of ab51266 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-SNRP70 antibody (ab51266)
This product has been referenced in:
- Luo X et al. Dynamic reorganization of the AC16 cardiomyocyte transcriptome in response to TNFa signaling revealed by integrated genomic analyses. BMC Genomics 15:155 (2014). WB . Read more (PubMed: 24564208) »
- Franklin S et al. Quantitative analysis of the chromatin proteome in disease reveals remodeling principles and identifies high mobility group protein B2 as a regulator of hypertrophic growth. Mol Cell Proteomics 11:M111.014258 (2012). WB . Read more (PubMed: 22270000) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"