Overview

  • Product name
  • Description
    Rabbit polyclonal to SNRPC
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Zebra finch, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 109-159 of Human SNRPC accoring to NP_003084.1.

  • Positive control
    • 293T, HeLa, Renca and TCMK-1 whole cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-SNRPC antibody (ab157116) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 17 kDa


    Exposure time: 3 minutes
  • All lanes : Anti-SNRPC antibody (ab157116) at 1 µg/ml

    Lane 1 : Renca whole cell lysate
    Lane 2 : TCMK whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 17 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation analysis of whole cell lysate from HeLa cells (1 mg for IP; 20% of IP loaded), labeling SNRPC with ab157116 at 6 µg/mg lysate (lane 1), control IgG (lane 2). For blotting immunoprecipitated SNRPC, ab157116 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

References

ab157116 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Nupage gel)
Sample
Mouse Cell lysate - whole cell (NSC34 cells)
Specification
NSC34 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Aug 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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