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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 14 kDa).
FunctionAppears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner.
Sequence similaritiesBelongs to the snRNP core protein family.
Post-translational modificationsMethylated on arginine residues by PRMT5 and PRMT7; methylation is required for assembly and biogenesis of snRNPs. Arg-97 is dimethylated, probably to asymmetric dimethylarginine.
small nuclear ribonucleoprotein D3 polypeptide 18kDa antibody
Small nuclear ribonucleoprotein Sm D3 antibody
snRNP core protein D3 antibody
Anti-SNRPD3 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-SNRPD3 antibody (ab111094)This image is courtesy of an Abreview submitted by Dr Kirk McManus
ab111094 staining HeLa cells by ICC/IF. The cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X100 in PBS. The cells were then stained with ab111094 at 1/500 in PBS for 1h at 22°C. An Alexa Fluro 488 goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody. Nuclei are stained in red with DAPI. SNRPD3 is observable in the cell's nuclei.
SNRPD3 contains a possible methylation site (SwissProt) which may explain its migration at a slightly higher molecular weight than predicted.
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab111094 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
References for Anti-SNRPD3 antibody (ab111094)
has not yet been referenced specifically in any publications.
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