Overview

  • Product name
  • Description
    Rabbit polyclonal to SNRPF
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide: SLPLNPKPFL NGLTGKPVMV KLKWGMEYKG YLVSVDGYMN MQLANTEEYI, corresponding to amino acids 2-51 of Human SNRPF

  • Positive control
    • Human heart. HepG2 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3125 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Images

  • All lanes : Anti-SNRPF antibody (ab66895) at 0.3125 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 10 kDa
    Observed band size : 10 kDa
    Additional bands at : 19 kDa. We are unsure as to the identity of these extra bands.Gel concentration 15%.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling SNRPF with ab66895 at 4-8µg/ml. Arrows indicate positively labelled myocardial cells. Magnification: 400X.

References

ab66895 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Total protein in input
3000 µg
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Verified customer

Submitted Jul 15 2014

Application
Western blot
Loading amount
2 µg
Gel Running Conditions
Reduced Denaturing (15% high TEMED)
Sample
Human Recombinant protein (E.coli)
Specification
E.coli
Treatment
recombinantly expressed as hetero-oligomer of SNRP E-F-G and purified
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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