Overview

  • Product name
  • Description
    Rabbit polyclonal to SNRPG
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Pig, Chimpanzee, Zebrafish, Macaque monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human SNRPG aa 1 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P62308

  • Positive control
    • This antibody gave a positive signal in HEK293 whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 8 kDa (predicted molecular weight: 8 kDa).

Target

Images

  • Anti-SNRPG antibody (ab111194) at 1 µg/ml (Milk blocking 3%) + HEK293 (Human) Whole Cell Lysate (ab52256) at 25 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 8 kDa
    Observed band size : 8 kDa
    Additional bands at : 36 kDa (possible non-specific binding),80 kDa (possible non-specific binding).

    Exposure time : 90 seconds

    This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab111194 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab111194 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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