Overview

  • Product name
  • Description
    Goat polyclonal to SNX1
  • Specificity
    This antibody is expected to react with isoform a and isoform c (NP_003090.2; NP_690039.1.).
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide:

    C-TNSSKPQPTYEELE

    , corresponding to internal sequence amino acids 122-135 of Human SNX1 (NP_003090.2; NP_690039.1).

  • Positive control
    • HeLa cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab99286 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 59 kDa). An approximately 75 kDa band is observed in Human lysates of the cell line HeLa. The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
ICC/IF Use a concentration of 3 - 5 µg/ml.

Target

  • Function
    May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
  • Sequence similarities
    Belongs to the sorting nexin family.
    Contains 1 BAR domain.
    Contains 1 PX (phox homology) domain.
  • Cellular localization
    Endosome membrane. Golgi apparatus > trans-Golgi network membrane. Early endosome membrane. Enriched on tubular elements of the early endosome membrane. Binds preferentially to highly curved membranes enriched in phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIn.
  • Information by UniProt
  • Database links
  • Alternative names
    • HsT17379 antibody
    • MGC8664 antibody
    • SNX 1 antibody
    • SNX 1a antibody
    • Snx1 antibody
    • SNX1_HUMAN antibody
    • SNX1A antibody
    • Sorting nexin 1 antibody
    • Sorting nexin 1A antibody
    • Sorting nexin-1 antibody
    • Vps5 antibody
    see all

Images

  • ab99286 staining SNX1 in SH5Y5Y cells by Immunocytochemistry/ Immunofluorescence. ab99286 diluted to 5 μg/ml showing vesicular staining.

  • 99286 (3.8µg/ml) staining of paraffin embedded Human Uterus tissue following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows vesicular staining in the cytoplasm of endometrial epithelium.

  • Anti-SNX1 antibody (ab99286) at 1 µg/ml + HeLa cell lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 59 kDa
    Primary incubation was 1 hour.

References

This product has been referenced in:
  • Marquer C  et al. Arf6 controls retromer traffic and intracellular cholesterol distribution via a phosphoinositide-based mechanism. Nat Commun 7:11919 (2016). Read more (PubMed: 27336679) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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