Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Sorting Nexin 1 encoded within exon 15 (LocusLink ID 6642).
Our Abpromise guarantee covers the use of ab995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|ICC||1/100 - 1/500.|
|WB||1/500 - 1/5000.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SNX1 with ab995 at 1/1000 (1µg/ml). Detection: DAB.
ab995 staining SNX1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.