SNX1 overexpression 293T lysate (whole cell) (ab94159)

Overview

  • Product nameSNX1 overexpression 293T lysate (whole cell)
    See all SNX1 lysates
  • Description
    SNX1 overexpression lysate
  • General notesab94159 is a 293T cell transfected lysate in which Human SNX1 has been transiently over-expressed using a pCMV-SNX1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Research areas
  • BackgroundFunction: May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Similarity: Belongs to the sorting nexin family. Contains 1 BAR domain. Contains 1 PX (phox homology) domain.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

SNX1 overexpression 293T lysate (whole cell) images

  • ab94159 at 15 µg/lane on an SDS-PAGE gel
  • All lanes : Anti-SNX1 antibody (ab55776) at 1/500 dilution

    Lane 1 : SNX1 overexpression 293T lysate (whole cell) (ab94159)
    Lane 2 : 293T non-transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for SNX1 overexpression 293T lysate (whole cell) (ab94159)

ab94159 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"