The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 39 kDa).Can be blocked with Human SNX16 peptide (ab22994). In transfected HEK293 transiently expressing SNX16 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293.
FunctionMay be involved in several stages of intracellular trafficking. Plays a role in protein transport from early to late endosomes. Plays a role in protein transport to the lysosome. Promotes degradation of EGFR after EGF signaling. Plays a role in intracellular transport of vesicular stomatitis virus nucleocapsids from the endosome to the cytoplasm.
Tissue specificityDetected in placenta, lung, liver,heart and pancreas.
Sequence similaritiesBelongs to the sorting nexin family. Contains 1 PX (phox homology) domain.
DomainThe PX domain mediates interaction with membranes enriched in phosphatidylinositol 3-phosphate.
Cellular localizationEarly endosome membrane. Late endosome membrane. Cytoplasm. Lysosome.