Overview

  • Product nameAnti-SNX18 antibody
    See all SNX18 primary antibodies
  • Description
    Rabbit polyclonal to SNX18
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 382-579 of Human SNX18 (AAH60791).

  • Positive control
    • A549 and HeLa cells. Cal27 xenograft tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab111702 is purified by antigen-affinity chromatography.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab111702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 69 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • FunctionMay be involved in several stages of intracellular trafficking. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Stimulates the GTPase activity of DNM2. Promotes DNM2 location at the plasma membrane.
  • Sequence similaritiesBelongs to the sorting nexin family.
    Contains 1 BAR domain.
    Contains 1 PX (phox homology) domain.
    Contains 1 SH3 domain.
  • DomainThe PX domain mediates interaction with membranes enriched in phosphatidylinositol-4,5-bisphosphate.
  • Cellular localizationEndomembrane system. Endosome membrane. Cell membrane. Localized at sites of endocytosis at the cell membrane. Detected on newly formed macropinosomes. Partially colocalized with clathrin and dynamin at the cell membrane. Transiently recruited to clathrin-coated pits at a late stage of clathrin-coated vesicle formation.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ11997 antibody
    • FLJ32560 antibody
    • FLJ61062 antibody
    • MGC150827 antibody
    • MGC150829 antibody
    • SH3 and PX domain containing protein 3B antibody
    • SH3 and PX domain-containing protein 3B antibody
    • SH3PX2 antibody
    • SH3PXD3B antibody
    • SNAG 1 antibody
    • SNAG1 antibody
    • SNX18 antibody
    • SNX18_HUMAN antibody
    • Sorting nexin 18 antibody
    • Sorting nexin associated golgi protein 1 antibody
    • Sorting nexin-18 antibody
    • Sorting nexin-associated Golgi protein 1 antibody
    see all

Anti-SNX18 antibody images

  • Anti-SNX18 antibody (ab111702) at 1/5000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size : 69 kDa
  • ab111702 at 1/200 dilution staining SNX18 in methanol-fixed HeLa cells. Lower image shows cells co-stained with Hoechst 33342 (blue).
  • ab111702 at 1/500 dilution staining SNX18 in Formalin-fixed, paraffin-embedded cal27 xenograft tissue.

References for Anti-SNX18 antibody (ab111702)

ab111702 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"