The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform Heat Induced Epitope Retrieval (HIER) at pH 6 using a pressure boiler as heat source.
FunctionMay be involved in several stages of intracellular trafficking.
Sequence similaritiesBelongs to the sorting nexin family. Contains 1 PX (phox homology) domain. Contains 1 PXA domain.
DomainThe PX domain mediates specific binding to membranes enriched in phosphatidylinositol-3-phosphate (PtdIns(P3)).