Overview

  • Product nameAnti-SNX2 antibody
    See all SNX2 primary antibodies
  • Description
    Chicken polyclonal to SNX2
  • Tested applicationsSuitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 12/138 of Human SNX2

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 58 kDa.
ICC 1/200.

Target

  • FunctionMay be involved in several stages of intracellular trafficking. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
  • Sequence similaritiesBelongs to the sorting nexin family.
    Contains 1 PX (phox homology) domain.
  • Cellular localizationEarly endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC5204 antibody
    • SNX 2 antibody
    • SNX2 antibody
    • SNX2_HUMAN antibody
    • Sorting nexin 2 antibody
    • Sorting nexin-2 antibody
    • Transformation related gene 9 protein antibody
    • Transformation-related gene 9 protein antibody
    • TRG 9 antibody
    • TRG-9 antibody
    • TRG9 antibody
    see all

Anti-SNX2 antibody images

  • Anti-SNX2 antibody (ab26201) at 1/2000 dilution + E coli derived fusion protein

    Secondary
    Goat anti IgY HRP

    Predicted band size : 58 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Subcellular localisation of SNX2 in human HeLa cells using ab26201 and an anti-chicken-Cy3 conjugated secondary antibody (Red). Nuclear staining with DAPI (Blue).

    See Abreview

  • All lanes : Anti-SNX2 antibody (ab26201) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP conjugated sheep anti-chicken IgG

    Predicted band size : 58 kDa
    Observed band size : ~60 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-SNX2 antibody (ab26201)

This product has been referenced in:
  • Spooner E  et al. Systematic screens for proteins that interact with the mucolipidosis type IV protein TRPML1. PLoS One 8:e56780 (2013). WB ; Mouse . Read more (PubMed: 23418601) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 25 µg
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

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Verified customer

Submitted Dec 21 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Jun 29 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"