Overview

  • Product nameAnti-SNX5 antibody
    See all SNX5 primary antibodies
  • Description
    Goat polyclonal to SNX5
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    SLLQSCIDLFKNN

    , corresponding to C terminal amino acids 392-404 of Human SNX5.

  • Positive control
    • Jurkat Lysate.
  • General notesGenBank Accession Number – NP_055241; NP_689413

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 6 µg/ml.
ICC/IF Use at an assay dependent dilution. PubMed: 20065094
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

Target

  • FunctionMay be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation.
  • Sequence similaritiesBelongs to the sorting nexin family.
    Contains 1 BAR domain.
    Contains 1 PX (phox homology) domain.
  • DomainThe PX domain mediates interaction with membranes enriched in phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 4,5-bisphosphate.
  • Cellular localizationEarly endosome membrane. Cell membrane. Cytoplasmic vesicle membrane. Cytoplasm. Cell projection > phagocytic cup. Cell projection > ruffle. Recruited to the plasma membrane after EGF stimulation, which leads to increased levels of phosphatidylinositol 3,4-bisphosphate (PdtIns(3,4)P2). Detected on macropinosomes. Targeted to membrane ruffles in response to EGFR stimulation.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0910001N05Rik antibody
    • 1810032P22Rik antibody
    • AU019504 antibody
    • D2Ertd52e antibody
    • FLJ10931 antibody
    • OTTHUMP00000030340 antibody
    • OTTHUMP00000062927 antibody
    • OTTMUSP00000003367 antibody
    • OTTMUSP00000003368 antibody
    • RP11-504H3.2 antibody
    • RP23-35E16.2 antibody
    • snoRNA MBI-43 antibody
    • SNX 5 antibody
    • SNX5 antibody
    • SNX5_HUMAN antibody
    • Sorting nexin 5 antibody
    • Sorting nexin-5 antibody
    see all

Anti-SNX5 antibody images

  • Anti-SNX5 antibody (ab5983) at 1 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
  • ab5983 at 4µg/ml, staining SNX5 in Human breast tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with pH 6 citrate buffer was performed, and the sample was stained with HRP.
  • HeLa cells were grown on coverslips, washed in warm PBS, fixed in warm PFA (3.7% in PBS, 10 minutes), then washed (2x, 5 minutes, PBS), permeabilized (4 minutes, 3% BSA, and 0.5% Triton X-100 in PBS), and blocked in blocking solution (3% BSA in PBS, 30 minutes). Antibody labeling was performed by inversion of coverslips on 20 µl blocking solution with primary or secondary antibodies (1–5 µg/ml).
  • Anti-SNX5 antibody (ab5983) at 1/500 dilution + whole cell lysate prepared from murine macrophages treated with INF gamma at 20 µg

    Secondary
    HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-SNX5 antibody (ab5983)

This product has been referenced in:
  • Steinberg F  et al. A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport. Nat Cell Biol 15:461-71 (2013). Human . Read more (PubMed: 23563491) »
  • Esk C  et al. The clathrin heavy chain isoform CHC22 functions in a novel endosomal sorting step. J Cell Biol 188:131-44 (2010). ICC/IF ; Human . Read more (PubMed: 20065094) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (macrophage)
Loading amount 20 µg
Specification macrophage
Treatment IFNgamma
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 01 2012

Thank you for your enquiry. I'm afraid we don't sell the immunogen/peptide for this antibody. Please contact us again if you have further questions. Best of luck with your research.

Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab5983. At this point I would like to make the following suggestions in order to help improve your results. Decrease the amount of secondary antibody that you ar...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"