The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
Snx6 is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein associates with the long isoform of the leptin receptor, the transforming growth factor-beta family of receptor serine-threonine kinases, and with receptor tyrosine kinases for platelet-derived growth factor, insulin, and epidermal growth factor. This protein may form oligomeric complexes with family member proteins through interactions of both the PX domain and the coiled coil regions of the molecules. Translocation of this protein from the cytoplasm to the nucleus occurs after binding to proviral integration site 1 protein. This gene results in two transcripts encoding two distinct isoforms.
Predicted band size : 48 kDa Western blot analysis of Hela (lane 1) NIHT3 (lane 2) and IHKE1 (lane 3) cell lysates (40µg/lane) labeling Snx6 with ab131010 at 1:1000. A HRP-conjugated Goat anti-rabbit IgG (1:1000) was used as the secondary antibody.
Western blot - Anti-Snx6 antibody (ab131010)
Anti-Snx6 antibody (ab131010) at 1 µg/ml + Human placenta lysate at 10 µg