Overview

  • Product name
  • Description
    Rabbit polyclonal to SOAT 1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Cynomolgus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human SOAT 1.

  • Positive control
    • This antibody gave a positive signal in the following lysates: Mouse Spleen Tissue; Ramos Whole Cell; RAW264.7 Whole Cell (data not shown).

Properties

Applications

Our Abpromise guarantee covers the use of ab72229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 65 kDa).

Target

  • Function
    Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase.
  • Sequence similarities
    Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACACT antibody
    • ACAT antibody
    • ACAT-1 antibody
    • ACAT1 antibody
    • Acyl coenzyme A cholesterol acyltransferase 1 antibody
    • Acyl-coenzyme A:cholesterol acyltransferase 1 antibody
    • Cholesterol acyltransferase 1 antibody
    • SOAT antibody
    • SOAT1 antibody
    • SOAT1_HUMAN antibody
    • STAT antibody
    • Sterol O acyltransferase 1 antibody
    • Sterol O-acyltransferase 1 antibody
    see all

Images

  • All lanes : Anti-SOAT 1 antibody (ab72229) at 1 µg/ml

    Lane 1 : Spleen (Mouse) Tissue Lysate
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 65 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References

ab72229 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab72229.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up