• Product name
  • Description
    Goat polyclonal to SOCS1
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide: VLRDYLSSFPFQI, corresponding to C terminal amino acids 199-211 of Human SOCS1 .

  • Positive control
    • Mouse Spleen Lysate
  • General notes
    Principal Names - SSI-1; JAK binding protein; JAB; SOCS1; SOCS-1; CIS1; SSI1 Official Gene Symbol - SOCS1 GenBank Accession Number – NP_003736 LocusLink ID - 8651



Our Abpromise guarantee covers the use of ab9870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30-35 kDa.Can be blocked with Human SOCS1 peptide (ab23167).
ICC/IF Use at an assay dependent concentration. PubMed: 21628332
Flow Cyt Use at an assay dependent concentration. PubMed: 23298838ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.


  • Function
    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2.
  • Tissue specificity
    Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • Domain
    The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box.
  • Information by UniProt
  • Database links
  • Alternative names
    • CISH 1 antibody
    • CISH1 antibody
    • Cytokine inducible SH2 protein 1 antibody
    • JAB antibody
    • JAK binding protein antibody
    • JAK-binding protein antibody
    • Janus kinase binding protein antibody
    • SOCS 1 antibody
    • SOCS-1 antibody
    • Socs1 antibody
    • SOCS1_HUMAN antibody
    • SSI 1 antibody
    • SSI-1 antibody
    • SSI1 antibody
    • STAT induced STAT inhibitor 1 antibody
    • STAT-induced STAT inhibitor 1 antibody
    • Suppressor of cytokine signaling 1 antibody
    • Supressor of cytokine signalling 1 antibody
    • TEC interacting protein 3 antibody
    • Tec-interacting protein 3 antibody
    • TIP 3 antibody
    • TIP-3 antibody
    • TIP3 antibody
    see all


  • ab9870 (2.5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • This picture was kindly supplied as part of the review submitted by Minh Nguyen.
  • HEK293 overexpressing SOCS1 and probed with ab9870 (mock transfection in lane B).
  • Anti-SOCS1 antibody (ab9870) at 2 µg/ml + Mouse Spleen Lysate in RIPA Buffer at 35 µg


This product has been referenced in:
  • Zimmer J  et al. Nuclear Localization of Suppressor of Cytokine Signaling-1 Regulates Local Immunity in the Lung. Front Immunol 7:514 (2016). Read more (PubMed: 27917175) »
  • Yan B  et al. Micro-ribonucleic acid 29b inhibits cell proliferation and invasion and enhances cell apoptosis and chemotherapy effects of cisplatin via targeting of DNMT3b and AKT3 in prostate cancer. Onco Targets Ther 8:557-65 (2015). WB . Read more (PubMed: 25784815) »

See all 7 Publications for this product

Customer reviews and Q&As

Western blot
Rabbit Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
35 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 30 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (monocyte-derived macrophage)
Loading amount
30 µg
monocyte-derived macrophage
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 17 2013

Thank you for contacting us.

To our knowledge, customised protocols are not required for this product, any general Flow Cytometry protocol could be used with this antibody.


Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Rat Cell lysate - whole cell (rat Bone marrow derived macrophages)
Loading amount
15 µg
rat Bone marrow derived macrophages
stimulants of macrophage activation for 4 hours
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Apr 04 2008

Thank you for your enquiry. The differences between goat polyclonal to SOCS1 (ab9870) and rabbit polyclonal to SOCS1 (ab3691) is that ab9870 reacts with mouse and is predicted to react with human due to sequence homology. So far it has only been sh...

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Thank you for your patience. As ab5923 was originated outside Abcam I had to contact the originator regarding your question and have just heard back. There was a mistake on the datasheet for ab5923 - it is a CIS1 antibody, not a SOCS1 antibody. I ap...

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I'm sorry to hear that you are having problems with these two antibodies. I am enquiring with the originators of these two antibodies for suggestions that will help you out. In the meantime, below is a general protocol for ab9870 that will be helpful f...

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