Our Abpromise guarantee covers the use of ab9870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30-35 kDa.Can be blocked with Human SOCS1 peptide (ab23167).|
|ICC/IF||Use at an assay dependent concentration. PubMed: 21628332|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 23298838ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.|