Anti-SOCS1 antibody (ab9870)
Key features and details
- Goat polyclonal to SOCS1
- Suitable for: Flow Cyt (Intra), ICC/IF, ICC, Flow Cyt, IHC-P
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-SOCS1 antibody
See all SOCS1 primary antibodies -
Description
Goat polyclonal to SOCS1 -
Host species
Goat -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, ICC, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig
Predicted to work with: Cow, Dog -
Immunogen
Synthetic peptide corresponding to Human SOCS1 aa 150-250 (C terminal).
Database link: NP_003736.1 -
Positive control
- Flow Cyt (Intra): HEK293 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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ICC/IF |
Use at an assay dependent concentration. PubMed: 21628332
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ICC |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration. PubMed: 23298838
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
Use a concentration of 2 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
ICC/IF
Use at an assay dependent concentration. PubMed: 21628332 |
ICC
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. PubMed: 23298838 ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 2 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. -
Tissue specificity
Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 SH2 domain.
Contains 1 SOCS box domain. -
Domain
The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box. - Information by UniProt
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Database links
- Entrez Gene: 8651 Human
- Entrez Gene: 12703 Mouse
- Entrez Gene: 252971 Rat
- Omim: 603597 Human
- SwissProt: O15524 Human
- SwissProt: O35716 Mouse
- SwissProt: Q9QX78 Rat
- Unigene: 50640 Human
see all -
Alternative names
- CIS1 antibody
- CISH 1 antibody
- CISH1 antibody
see all
Images
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Flow cytometric analysis of HEK293 cells with ab9870 (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of paraffin embedded Human Kidney with ab9870 (8 µg/ml). Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of paraffin embedded Mouse Kidney with ab9870 (8 µg/ml). Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
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Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation with ab9870 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
Protocols
Datasheets and documents
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Datasheet download
References (31)
ab9870 has been referenced in 31 publications.
- Xu L et al. FABP4 activates the JAK2/STAT2 pathway via Rap1a in the homocysteine-induced macrophage inflammatory response in ApoE-/- mice atherosclerosis. Lab Invest 102:25-37 (2022). PubMed: 34725437
- Suñer C et al. Macrophage inflammation resolution requires CPEB4-directed offsetting of mRNA degradation. Elife 11:N/A (2022). PubMed: 35442882
- Li L et al. Effect of Platycodon grandiflorus Polysaccharide on M1 Polarization Induced by Autophagy Degradation of SOCS1/2 Proteins in 3D4/21 Cells. Front Immunol 13:934084 (2022). PubMed: 35844489
- Guo Q et al. SOCS1 Mediates Berberine-Induced Amelioration of Microglial Activated States in N9 Microglia Exposed to β Amyloid. Biomed Res Int 2021:9311855 (2021). PubMed: 34778460
- Shirai Y et al. A novel de novo truncating TRIM8 variant associated with childhood-onset focal segmental glomerulosclerosis without epileptic encephalopathy: a case report. BMC Nephrol 22:417 (2021). PubMed: 34930159