Overview

  • Product nameAnti-SOCS2 antibody
    See all SOCS2 primary antibodies
  • Description
    Rabbit polyclonal to SOCS2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human SOCS2 .

    (Peptide available as ab66732.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HepG2, HeLa, SW480, Raji, MOLT4 and in Mouse Thymus tissue lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab66733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).Can be blocked with Human SOCS2 peptide (ab66732).

Target

  • FunctionSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • Tissue specificityHigh expression in heart, placenta, lung, kidney and prostate.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • DomainThe SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIS 2 antibody
    • CIS-2 antibody
    • CIS2 antibody
    • Cish 2 antibody
    • Cish2 antibody
    • Cytokine inducible SH2 protein 2 antibody
    • Cytokine-inducible SH2 protein 2 antibody
    • SOCS 2 antibody
    • SOCS-2 antibody
    • Socs2 antibody
    • SOCS2_HUMAN antibody
    • SSI 2 antibody
    • SSI-2 antibody
    • SSI2 antibody
    • STAT induced STAT inhibitor 2 antibody
    • STAT-induced STAT inhibitor 2 antibody
    • STATI 2 antibody
    • STATI2 antibody
    • Suppressor of cytokine signaling 2 antibody
    see all

Anti-SOCS2 antibody images

  • All lanes : Anti-SOCS2 antibody (ab66733) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : SW480 whole cell lysate (ab3957)
    Lane 4 : Raji whole cell lysate (ab30124)
    Lane 5 : Mouse thymus tissue lysate - total protein (ab29285)
    Lane 6 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 22 kDa

References for Anti-SOCS2 antibody (ab66733)

ab66733 has not yet been referenced specifically in any publications.

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