Overview

  • Product name
  • Description
    Rabbit polyclonal to SOCS2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human SOCS2 .

    (Peptide available as ab66732.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HepG2, HeLa, SW480, Raji, MOLT4 and in Mouse Thymus tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab66733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).Can be blocked with Human SOCS2 peptide (ab66732).

Target

  • Function
    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • Tissue specificity
    High expression in heart, placenta, lung, kidney and prostate.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • Domain
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIS 2 antibody
    • CIS-2 antibody
    • CIS2 antibody
    • Cish 2 antibody
    • Cish2 antibody
    • Cytokine inducible SH2 protein 2 antibody
    • Cytokine-inducible SH2 protein 2 antibody
    • SOCS 2 antibody
    • SOCS-2 antibody
    • Socs2 antibody
    • SOCS2_HUMAN antibody
    • SSI 2 antibody
    • SSI-2 antibody
    • SSI2 antibody
    • STAT induced STAT inhibitor 2 antibody
    • STAT-induced STAT inhibitor 2 antibody
    • STATI 2 antibody
    • STATI2 antibody
    • Suppressor of cytokine signaling 2 antibody
    see all

Images

  • All lanes : Anti-SOCS2 antibody (ab66733) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : SW480 whole cell lysate (ab3957)
    Lane 4 : Raji whole cell lysate (ab30124)
    Lane 5 : Mouse thymus tissue lysate - total protein (ab29285)
    Lane 6 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 22 kDa

References

ab66733 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab66733.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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