Overview

  • Product name
  • Description
    Rabbit polyclonal to SOCS2
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to the C terminal region of human SOCS2.

  • Positive control
    • Human pancreas IF/ICC: HepG2 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab74533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
ICC/IF
  • Application notes
    IHC-P: 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
    • Tissue specificity
      High expression in heart, placenta, lung, kidney and prostate.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Contains 1 SH2 domain.
      Contains 1 SOCS box domain.
    • Domain
      The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
    • Information by UniProt
    • Database links
    • Alternative names
      • CIS 2 antibody
      • CIS-2 antibody
      • CIS2 antibody
      • Cish 2 antibody
      • Cish2 antibody
      • Cytokine inducible SH2 protein 2 antibody
      • Cytokine-inducible SH2 protein 2 antibody
      • SOCS 2 antibody
      • SOCS-2 antibody
      • Socs2 antibody
      • SOCS2_HUMAN antibody
      • SSI 2 antibody
      • SSI-2 antibody
      • SSI2 antibody
      • STAT induced STAT inhibitor 2 antibody
      • STAT-induced STAT inhibitor 2 antibody
      • STATI 2 antibody
      • STATI2 antibody
      • Suppressor of cytokine signaling 2 antibody
      see all

    Anti-SOCS2 antibody images

    • ICC/IF image of ab74533 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74533, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Human pancreas stained with ab74533 at 1/100 dilution.

    References for Anti-SOCS2 antibody (ab74533)

    This product has been referenced in:
    • Li B  et al. Development and Validation of a Three-gene Prognostic Signature for Patients with Hepatocellular Carcinoma. Sci Rep 7:5517 (2017). IHC . Read more (PubMed: 28717245) »
    • Iglesias-Gato D  et al. SOCS2 mediates the cross talk between androgen and growth hormone signaling in prostate cancer. Carcinogenesis N/A:N/A (2013). Read more (PubMed: 24031028) »

    See all 3 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cat Tissue sections (spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
    Permeabilization
    No
    Specification
    spleen
    Blocking step
    (agent) for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 06 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: tris-EDTA, pH9.0
    Permeabilization
    No
    Specification
    liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 03 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Dog Tissue sections (liver)
    Permeabilization
    No
    Specification
    liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    Submitted Feb 19 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (nerve)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
    Permeabilization
    No
    Specification
    nerve
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 22 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer, pH6.0
    Sample
    Rat Tissue sections (sciatic nerve)
    Specification
    sciatic nerve
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 28 2014

    Merci pour votre patience.

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