Anti-SOCS2 antibody (ab92847)

Overview

  • Product nameAnti-SOCS2 antibody
    See all SOCS2 primary antibodies
  • Description
    Rabbit polyclonal to SOCS2
  • Tested applicationsSuitable for: WB, IP, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to the C-terminus of SOCS2 protein from Human, Mouse and Rat origins.

  • Positive control
    • Mouse liver and muscle cell lysates. IHC-P: Human lung FFPE tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab92847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ELISA
IHC-P
  • Application notesELISA: Use at a concentration of 0.01 - 0.1 µg/ml.
    IHC-P: Use at a concentration of 0.5 - 2 µg/ml.
    IP: Use at a concentration of 2-5 µg/ml.
    WB: Use at a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 22 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
    • Tissue specificityHigh expression in heart, placenta, lung, kidney and prostate.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesContains 1 SH2 domain.
      Contains 1 SOCS box domain.
    • DomainThe SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
    • Information by UniProt
    • Database links
    • Alternative names
      • CIS 2 antibody
      • CIS-2 antibody
      • CIS2 antibody
      • Cish 2 antibody
      • Cish2 antibody
      • Cytokine inducible SH2 protein 2 antibody
      • Cytokine-inducible SH2 protein 2 antibody
      • SOCS 2 antibody
      • SOCS-2 antibody
      • Socs2 antibody
      • SOCS2_HUMAN antibody
      • SSI 2 antibody
      • SSI-2 antibody
      • SSI2 antibody
      • STAT induced STAT inhibitor 2 antibody
      • STAT-induced STAT inhibitor 2 antibody
      • STATI 2 antibody
      • STATI2 antibody
      • Suppressor of cytokine signaling 2 antibody
      see all

    Anti-SOCS2 antibody images

    • IHC image of SOCS2 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92847, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • All lanes : Anti-SOCS2 antibody (ab92847) at 1/500 dilution

      Lane 1 : Mouse liver cell Lysate
      Lane 2 : Mouse muscle cell Lysate


      Predicted band size : 22 kDa
      Observed band size : 22 kDa

    References for Anti-SOCS2 antibody (ab92847)

    ab92847 has not yet been referenced specifically in any publications.

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (tumor colon cells)
    Loading amount 25 µg
    Specification tumor colon cells
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Sep 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"