Overview

  • Product nameAnti-SOCS3 antibody
    See all SOCS3 primary antibodies
  • Description
    Rabbit polyclonal to SOCS3
  • Tested applicationsSuitable for: WB, IP, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to the C-terminus of SOCS3 protein of Human, Mouse and Rat origin.

  • Positive control
    • Mouse liver or muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab92860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ELISA
IHC-P
ICC/IF
  • Application notesELISA: Use at a concentration of 0.01 - 0.1 µg/ml.
    IHC-P: Use at a concentration of 0.5 - 2 µg/ml.
    IP: Use at a concentration of 2 - 5 µg/ml.
    WB: Use at a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 25 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
    • Tissue specificityWidely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
    • PathwayProtein modification; protein ubiquitination.
    • Involvement in diseaseNote=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
    • Sequence similaritiesContains 1 SH2 domain.
      Contains 1 SOCS box domain.
    • DomainThe ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
      The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATOD4 antibody
      • CIS 3 antibody
      • CIS-3 antibody
      • CIS3 antibody
      • Cish3 antibody
      • Cytokine induced SH2 protein 3 antibody
      • Cytokine-inducible SH2 protein 3 antibody
      • E2a Pbx1 target gene in fibroblasts 10 antibody
      • EF 10 antibody
      • MGC71791 antibody
      • SOCS 3 antibody
      • SOCS-3 antibody
      • Socs3 antibody
      • SOCS3_HUMAN antibody
      • SSI 3 antibody
      • SSI-3 antibody
      • SSI3 antibody
      • STAT induced STAT inhibitor 3 antibody
      • STAT-induced STAT inhibitor 3 antibody
      • Suppressor of cytokine signaling 3 antibody
      see all

    Anti-SOCS3 antibody images

    References for Anti-SOCS3 antibody (ab92860)

    ab92860 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (murine embryonic fibroblasts)
    Specification murine embryonic fibroblasts
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.2% Triton-X-100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
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    Submitted Dec 17 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"