Overview

  • Product nameAnti-SOCS4 antibody
    See all SOCS4 primary antibodies
  • Description
    Rabbit polyclonal to SOCS4
  • SpecificityThis antibodyreacts predominantly with SOCS 4. The antibodies wereevaluated for specificity with a dot blot assay using synthetically prepared SOCS peptides. It onlyrecognizes SOCS 4, not other SOCS.
  • Tested applicationsSuitable for: IHC-P, WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to C-terminus of human/ mouse SOCS 4.

  • General notes


    Accumulating evidence demonstrates that cytokine receptorsignaling is negatively regulated by a family of Srchomology 2 domain-containing adaptor molecules Termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family have beenrecognized, they are SOCS 1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an Nterminalregion of variable length and amino acidcomposition, a central SH2 domain, and a previously unrecognized C-terminal motif that we have called the SOCS box. The SOCS proteins appear to form part of aclassical negative feed back loop that regulates cytokinesignal transduction via a STAT-induced transcriptionalmechanism. Transcription of each of the SOCS genesoccurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. Within the SOCS subfamily, SOCS 4, SOCS 5, SOCS 6 and SOCS 7 remain poorly understood. On the basis ofstructural considerations, it would be expected if SOCS 4 and SOCS 7, like SOCS 1, SOCS 2, SOCS 3 and CIS, acted tonegatively regulated signal transduction, but not surewhich specific cytokine is involved.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPBS with 0.02% sodium azide
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesAccumulating evidence demonstrates that cytokine receptorsignaling is negatively regulated by a family of Srchomology 2 domain-containing adaptor molecules Termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family have beenrecognized, they are SOCS 1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an Nterminalregion of variable length and amino acidcomposition, a central SH2 domain, and a previously unrecognized C-terminal motif that we have called the SOCS box. The SOCS proteins appear to form part of aclassical negative feed back loop that regulates cytokinesignal transduction via a STAT-induced transcriptionalmechanism. Transcription of each of the SOCS genesoccurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. Within the SOCS subfamily, SOCS 4, SOCS 5, SOCS 6 and SOCS 7 remain poorly understood. On the basis ofstructural considerations, it would be expected if SOCS 4 and SOCS 7, like SOCS 1, SOCS 2, SOCS 3 and CIS, acted tonegatively regulated signal transduction, but not surewhich specific cytokine is involved.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IP Use at an assay dependent concentration. use at a concentration of 3.0 - 5.0 µg / extract from 107 cells.
ELISA Use a concentration of 0.1 - 1 µg/ml.

Target

  • FunctionSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • DomainThe SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIS4 antibody
    • Cish4 antibody
    • Cytokine inducible SH2 protein 4 antibody
    • DKFZp686J1568 antibody
    • HSPC060 antibody
    • SH2 domain containing SOCS box protein antibody
    • SOCS-4 antibody
    • SOCS-7 antibody
    • SOCS4 antibody
    • SOCS4_HUMAN antibody
    • SOCS6 antibody
    • SOCS7 antibody
    • SSI4 antibody
    • STAI4 antibody
    • STAT induced STAT inhibitor 4 antibody
    • STAT4 antibody
    • STATI4 antibody
    • Suppressor of cytokine signaling 4 antibody
    • Suppressor of cytokine signaling 7 antibody
    see all

References for Anti-SOCS4 antibody (ab3694)

This product has been referenced in:
  • Sutherland JM  et al. Suppressor of cytokine signaling 4 (SOCS4): moderator of ovarian primordial follicle activation. J Cell Physiol 227:1188-98 (2012). WB, IHC-P ; Mouse . Read more (PubMed: 21604262) »
  • Kobayashi D  et al. Suppressor of cytokine signaling 4 detected as a novel gastric cancer suppressor gene using double combination array analysis. World J Surg 36:362-72 (2012). WB ; Human . Read more (PubMed: 22127425) »

See all 2 Publications for this product

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