SOCS4 overexpression 293T lysate (whole cell) (ab94295)
Overview
-
Product name
SOCS4 overexpression 293T lysate (whole cell)
See all SOCS4 lysates -
General notes
ab94295 is a 293T cell transfected lysate in which Human SOCS4 has been transiently over-expressed using a pCMV-SOCS4 plasmid. The lysate is provided in 1 x Sample Buffer.
-
Tested applications
Suitable for: WBmore details
Properties
-
Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Research areas
-
Background
Function: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Pathway: Protein modification; protein ubiquitination. Similarity: Contains 1 SH2 domain. Contains 1 SOCS box domain. Domain: The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent dilution.
|
Notes |
---|
WB
Use at an assay dependent dilution. |
Images
-
ab94295 at 15µg/lane on an SDS-PAGE gel.
-
All lanes : Anti-SOCS4 antibody (ab57337) at 1/500 dilution
Lane 1 :SOCS4 overexpression 293T lysate (whole cell) (ab94295)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab94295 has not yet been referenced specifically in any publications.