SOCS4 overexpression 293T lysate (whole cell) (ab94295)

Overview

  • Product nameSOCS4 overexpression 293T lysate (whole cell)
  • Description
    SOCS4 overexpression lysate
  • General notesab94295 is a 293T cell transfected lysate in which Human SOCS4 has been transiently over-expressed using a pCMV-SOCS4 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Research areas
  • BackgroundFunction: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Pathway: Protein modification; protein ubiquitination. Similarity: Contains 1 SH2 domain. Contains 1 SOCS box domain. Domain: The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.

Applications

Our Abpromise guarantee covers the use of ab94295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

SOCS4 overexpression 293T lysate (whole cell) images

  • ab94295 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-SOCS4 antibody (ab57337) at 1/500 dilution

    Lane 1 : SOCS4 overexpression 293T lysate (whole cell) (ab94295)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for SOCS4 overexpression 293T lysate (whole cell) (ab94295)

ab94295 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"