• Product name
  • Description
    Rabbit polyclonal to SOCS5
  • Specificity
    This antibody reacts predominantly with SOCS 5. It was evaluated for specificity with a dot blot assay using synthetically prepared SOCS peptides: it only recognizes SOCS 5, no other SOCS.
  • Tested applications
    Suitable for: IHC-P, WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to C-terminus of human/ mouse SOCS 5.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    PBS with 0.02% sodium azide
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab3695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
IP Use 3-5µg for 107 cells.
ELISA Use a concentration of 0.1 - 1 µg/ml.


  • Relevance
    The eight members of the recently identified Suppressor of Cytokines Signaling (SOCS) family are SOCS1, SOCS2, SOCS3, SOCS4, SOCS5, SOCS6, SOCS7, and CIS. Structurally the SOCS proteins are composed of an N- terminal region of variable length and amino acid composition, a central SH2 domain, and a C-terminal motif called the SOCS box. The SOCS proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. During Th1 differentiation a reduction in the association of Jak1 with the IL4 Receptor correlated with the appearance of SOCS5. SOCS5 protein was preferentially expressed in committed Th1 cells and interacted with the cytoplasmic region of the IL4 Receptor alpha chain irrespective of receptor tyrosine phosphorylation. This unconventional interaction of SOCS5 protein with IL4 Receptor resulted in the inhibition of IL4-mediated signal transducer and activator of transcription-6 activation. T cells from transgenic mice constitutively expressing SOCS5 exhibited a significant reduction of IL4-mediated Th2 development. Therefore, the induced SOCS5 protein in Th1 differentiation environment may play an important role by regulating Th1 and Th2 balance.
  • Database links
  • Alternative names
    • CIS-6 antibody
    • CIS6 antibody
    • CISH5 antibody
    • CISH6 antibody
    • Cytokine inducible SH2 containing protein 5 antibody
    • Cytokine inducible SH2 protein 6 antibody
    • KIAA0671 antibody
    • SOCS-5 antibody
    • Suppressor of cytokine signaling 5 antibody
    see all


  • ab3695 staining formalin-fixed, paraffin embeded tissue section of cardiac myocytes within human heart. The primary antibody was diluted 10 μg/ml and it was detected by ABC kit. Nuclears are counterstained with hematoxylin purple.


This product has been referenced in:
  • Yan B  et al. Micro-ribonucleic acid 29b inhibits cell proliferation and invasion and enhances cell apoptosis and chemotherapy effects of cisplatin via targeting of DNMT3b and AKT3 in prostate cancer. Onco Targets Ther 8:557-65 (2015). WB . Read more (PubMed: 25784815) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us for technical support with ab3695. I would appreciate you clarifying why you have added a claim report to the protocol questionnaire, as the claim report is not our Abcam branded report, can I please make sure that when a cu...

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