Overview

  • Product nameAnti-SOCS5 antibody
    See all SOCS5 primary antibodies
  • Description
    Rabbit polyclonal to SOCS5
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide containing a sequence corresponding to a region within amino acids 2-12 (

    DKVGKMWNNFK

    ) of Human SOCS5 (NP_054730).

  • Positive control
    • Molt-4 whole cell lysate; Ca922; 293T, A431, H1299, HeLa, HepG2, and Raji cell lysates

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 40% Glycerol, 1X PBS, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab97283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/500.

Target

  • RelevanceThe eight members of the recently identified Suppressor of Cytokines Signaling (SOCS) family are SOCS1, SOCS2, SOCS3, SOCS4, SOCS5, SOCS6, SOCS7, and CIS. Structurally the SOCS proteins are composed of an N- terminal region of variable length and amino acid composition, a central SH2 domain, and a C-terminal motif called the SOCS box. The SOCS proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. During Th1 differentiation a reduction in the association of Jak1 with the IL4 Receptor correlated with the appearance of SOCS5. SOCS5 protein was preferentially expressed in committed Th1 cells and interacted with the cytoplasmic region of the IL4 Receptor alpha chain irrespective of receptor tyrosine phosphorylation. This unconventional interaction of SOCS5 protein with IL4 Receptor resulted in the inhibition of IL4-mediated signal transducer and activator of transcription-6 activation. T cells from transgenic mice constitutively expressing SOCS5 exhibited a significant reduction of IL4-mediated Th2 development. Therefore, the induced SOCS5 protein in Th1 differentiation environment may play an important role by regulating Th1 and Th2 balance.
  • Database links
  • Alternative names
    • CIS-6 antibody
    • CIS6 antibody
    • CISH5 antibody
    • CISH6 antibody
    • Cytokine inducible SH2 containing protein 5 antibody
    • Cytokine inducible SH2 protein 6 antibody
    • KIAA0671 antibody
    • SOCS-5 antibody
    • Suppressor of cytokine signaling 5 antibody
    see all

Anti-SOCS5 antibody images

  • Anti-SOCS5 antibody (ab97283) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg

    Predicted band size : 61 kDa
  • Immunohistochemical analysis of SOCS5 in paraffin-embedded Ca922, using ab97283 at 1/100 dilution.

References for Anti-SOCS5 antibody (ab97283)

ab97283 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab97283.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"