Anti-SOD2 antibody [9E2BD2] (ab110300)

  Flow Cytometry - Superoxide Dismutase 2 antibody [9E2BD2] (ab110300)

HL-60 cells were stained with 1 µg/mL ab110300 in blue or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

  Immunocytochemistry/ Immunofluorescence - Superoxide Dismutase 2 antibody [9E2BD2] (ab110300)

Immunocytochemistry image of Superoxide Dismutase 2 stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110300 at 5 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Superoxide Dismutase 2 antibody [9E2BD2] (ab110300)

Immunohistochemistry of Superoxide Dismutase 2 in Human cerebellum visualized with ab110300. Superoxide Dismutase 2 immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. Note the distinctive subcellular localization of Superoxide Dismutase 2 immunoreactivity in the Purkinje cell bodies.