• Product nameAnti-SOD2/MnSOD antibody [9E2BD2]
    See all SOD2/MnSOD primary antibodies
  • Description
    Mouse monoclonal [9E2BD2] to SOD2/MnSOD
  • Tested applicationsSuitable for: ICC/IF, IHC-P, IP, In-Cell ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Purified proteins from bovine liver, bovine heart, porcine heart

  • Positive control
    • Human HDFn and HL-60 cells and Human cerebellum.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.


Associated products


Our Abpromise guarantee covers the use of ab110300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. use 1mmol EDTA pH8
IP Use at an assay dependent concentration.
In-Cell ELISA Use a concentration of 4 µg/ml. 0.4 µg/well
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionDestroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
  • Involvement in diseaseGenetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similaritiesBelongs to the iron/manganese superoxide dismutase family.
  • Post-translational
    Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Indophenoloxidase B antibody
    • IPO B antibody
    • IPOB antibody
    • Manganese containing superoxide dismutase antibody
    • Manganese SOD antibody
    • Manganese superoxide dismutase antibody
    • Mangano superoxide dismutase antibody
    • Mn SOD antibody
    • Mn superoxide dismutase antibody
    • MNSOD antibody
    • MVCD6 antibody
    • SOD 2 antibody
    • SOD2 antibody
    • SODM_HUMAN antibody
    • Superoxide dismutase [Mn] mitochondrial antibody
    • Superoxide dismutase [Mn], mitochondrial antibody
    • Superoxide dismutase 2 mitochondrial antibody
    see all

Anti-SOD2/MnSOD antibody [9E2BD2] images

  • HL-60 cells were stained with 1 µg/mL ab110300 in blue or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Immunocytochemistry image of Superoxide Dismutase 2 stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110300 at 5 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
  • Immunohistochemistry of Superoxide Dismutase 2 in Human cerebellum visualized with ab110300. Superoxide Dismutase 2 immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. Note the distinctive subcellular localization of Superoxide Dismutase 2 immunoreactivity in the Purkinje cell bodies.

References for Anti-SOD2/MnSOD antibody [9E2BD2] (ab110300)

This product has been referenced in:
  • Zhang C  et al. Heteroplasmic mutations of the mitochondrial genome cause paradoxical effects on mitochondrial functions. FASEB J : (2012). WB ; Human . Read more (PubMed: 22925728) »

See 1 Publication for this product

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The lab sent me the following information:

The customer should use this protocol: http://www.mitosciences.com/PDF/ip.pdf
As in this protocol we would recommend conjugating the antibody to the prote...

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Thank you for contacting us. The cells were fixed in PFA 4% for 15 minutes. For permeabilization 90% methanol was used at -20 degrees Celcius for 30 minutes. Alternatively 0.1% Triton-X may be used and optimized. Alexa 488 was used as the s...

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