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Purified proteins from bovine liver, bovine heart, porcine heart
Our Abpromise guarantee covers the use of ab110300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. use 1mmol EDTA pH8|
|IP||Use at an assay dependent concentration.|
|In-Cell ELISA||Use a concentration of 4 µg/ml. 0.4 µg/well|
|Flow Cyt||Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"