Overview

  • Product nameAnti-SOD2/MnSOD antibody
    See all SOD2/MnSOD primary antibodies
  • Description
    Rabbit polyclonal to SOD2/MnSOD
  • Tested applicationsSuitable for: ELISA, IHC-Fr, ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Monkey, Gerbil
  • Immunogen

    Full length protein corresponding to Human SOD2/MnSOD.

  • Positive control
    • Rat brain tissue extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab13533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/150.
IHC-P Use at an assay dependent concentration.
WB 1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 26.6 kDa).
IP Use a concentration of 10 µg/ml.

Target

  • FunctionDestroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
  • Involvement in diseaseGenetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similaritiesBelongs to the iron/manganese superoxide dismutase family.
  • Post-translational
    modifications
    Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Indophenoloxidase B antibody
    • IPO B antibody
    • IPOB antibody
    • Manganese containing superoxide dismutase antibody
    • Manganese SOD antibody
    • Manganese superoxide dismutase antibody
    • Mangano superoxide dismutase antibody
    • Mn SOD antibody
    • Mn superoxide dismutase antibody
    • MNSOD antibody
    • MVCD6 antibody
    • SOD 2 antibody
    • SOD2 antibody
    • SODM_HUMAN antibody
    • Superoxide dismutase [Mn] mitochondrial antibody
    • Superoxide dismutase [Mn], mitochondrial antibody
    • Superoxide dismutase 2 mitochondrial antibody
    see all

Anti-SOD2/MnSOD antibody images

  • All lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution

    Lane 1 : mouse liver whole cell lysate
    Lane 2 : mouse hepatocytes whole cell lysate

    Secondary
    Goat anti-rabbit HRP conjugate at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26.6 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)


    Exposure time : 15 seconds
  • ab13533 staining Superoxide Dismutase 2 in Human normal fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% BSA for 1 hour at 37°C. Samples were incubated with primary antibody (1/150 in 1% BSA) for 2 hours at 37°C. An undiluted Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody. Nuclei were counterstained with DAPI.

    See Abreview

  • ab13533 at 1/100 dilution staining Superoxide Dismutase in rat muscle fibre tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A goat anti-rabbit secondary was used at 1/10 dilution.

  • All lanes : Anti-SOD2/MnSOD antibody (ab13533) at 1/5000 dilution

    Lane 1 : 2ug mouse liver homogenate supernatant
    Lane 2 : 1ug mouse liver homogenate supernatant
    Lane 3 : 0.5ug mouse liver homogenate supernatant

    Secondary
    HRP conjugated donkey anti-rabbit antibody

    Performed under reducing conditions.

    Predicted band size : 26.6 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds

    This image is courtesy of an Abreview submitted by Dr sandra sobocanec

    See Abreview

  • ab13533 staining Superoxide Dismutase 2 in human Hella cells by ICC/IF. Cells were acetone fixed and blocked in 5% serum for 1 hour at 24°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 24°C. The secondary antibody used was Alexa Fluor®594 donkey anti-rabbit diluted at 1/100.

    See Abreview

  • ab13533 staining NGF-differentiated PC12 cells (rat) by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C.  The primary antibody was diluted to 2µg/ml in PBS and incubated with the sample for 1 hour at 25°C.  An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

References for Anti-SOD2/MnSOD antibody (ab13533)

This product has been referenced in:
  • Carlson RI  et al. Development and application of an antibody-based protein microarray to assess physiological stress in grizzly bears (Ursus arctos). Conserv Physiol 4:cow001 (2016). Other . Read more (PubMed: 27293753) »
  • Greco T  et al. Ketogenic diet decreases oxidative stress and improves mitochondrial respiratory complex activity. J Cereb Blood Flow Metab 36:1603-13 (2016). Read more (PubMed: 26661201) »

See all 30 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Epithelial OVCA cells)
Gel Running Conditions Reduced Denaturing (12.5%)
Loading amount 75 µg
Treatment 0- 100µM Perifosine 24 hr
Specification Epithelial OVCA cells
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 09 2016

Application Western blot
Sample Rat Tissue lysate - whole (Bone tissue (Tibia))
Gel Running Conditions Reduced Denaturing (12,5%)
Loading amount 50 µg
Specification Bone tissue (Tibia)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Aug 25 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Rat gonad)
Antigen retrieval step Enzymatic
Permeabilization No
Specification Rat gonad
Blocking step Serum as blocking agent for 12 hour(s) and 45 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 14 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (liver, hepatocytes)
Specification liver, hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 05 2015

Application Western blot
Loading amount 80 µg
Gel Running Conditions Reduced Denaturing (12.5% Acrylamide, 2D gel ph 3-10)
Sample Pig Tissue lysate - other (Muscle Sarcoplasm)
Specification Muscle Sarcoplasm
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Shannon Cruzen

Verified customer

Submitted Sep 29 2014

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 02 2014

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (4-20%)
Sample Mouse Tissue lysate - whole (Lung)
Specification Lung
Treatment S. aureus 10^7 CFU
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Alan Chang

Verified customer

Submitted Mar 21 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% separating gel)
Sample Rat Tissue lysate - whole (cerebellum)
Specification cerebellum
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 21 2014

Application Western blot
Loading amount 20000 cells
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (jurkats)
Specification jurkats
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Dr. Tejpal Gill

Verified customer

Submitted Jan 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 75 µg
Gel Running Conditions Reduced Denaturing (12.5)
Sample Rat Cell lysate - other (ovary)
Specification ovary
Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 11 2013

1-10 of 35 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"