Overview

  • Product name
  • Description
    Rabbit polyclonal to SOD2/MnSOD
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, ELISA, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Monkey
  • Immunogen

    Full length protein corresponding to Rat SOD2/MnSOD.

  • Positive control
    • ICC/IF: HeLa cells. IHC-P: Mouse back skin tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab13534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use a concentration of 2 µg/ml.
ELISA Use at an assay dependent concentration.
IHC-P 1/100.
WB 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 26.6 kDa).
IP Use a concentration of 10 µg/ml.

Target

  • Function
    Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
  • Involvement in disease
    Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities
    Belongs to the iron/manganese superoxide dismutase family.
  • Post-translational
    modifications
    Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Indophenoloxidase B antibody
    • IPO B antibody
    • IPOB antibody
    • Manganese containing superoxide dismutase antibody
    • Manganese SOD antibody
    • Manganese superoxide dismutase antibody
    • Mangano superoxide dismutase antibody
    • Mn SOD antibody
    • Mn superoxide dismutase antibody
    • MNSOD antibody
    • MVCD6 antibody
    • SOD 2 antibody
    • SOD2 antibody
    • SODM_HUMAN antibody
    • Superoxide dismutase [Mn] mitochondrial antibody
    • Superoxide dismutase [Mn], mitochondrial antibody
    • Superoxide dismutase 2 mitochondrial antibody
    see all

Anti-SOD2/MnSOD antibody images



  • Predicted band size : 26.6 kDa
    Observed band size : 22.5 kDa (why is the actual band size different from the predicted?)

    Image courtesy of an anonymous Abreview.

    Primary antibody was incubated for 16 hours at 4°C, at a 1/2000 dilution.Secondary antibody was a goat anti-rabbit conjugated to Alexa Fluor® 680, used at a 1/5000 dilution.

    See Abreview

  • ab13534 at 1/100 dilution staining Superoxide Dismutase 2 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/50 dilution.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SOD2/MnSOD with ab13534. Cells were fixed with 2% formaldehyde for 20 minutes at room temperature and incubated with the primary antibody at a dilution of 1/120 for 12 hours at 4°C. Followed by incubation with a R-PE-conjugated goat anti-rabbit IgG at a dilution of 1/200 for 2 hours at room temperature. Counterstained with DAPI (blue) at 1/40000 for 2 hours at room temperature. Magnification: 100x.

    Left: DAPI (blue) nuclear stain. Middle: SOD2/MnSOD. Right: Merge.

  • ab13534 staining SOD2 in Human colon and Horse Ileo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/2000) for 18 hours.

    See Abreview

  • ab13534 staining differentiating (rat) PC12 cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton X100 prior to blocking in 10% serum at RT for 1 hour.  The primary antibody was incubated with the sample for 1 hour at 2 µg/ml.  An Alexa Fluor® 488 conjugated goat anti-rabbit antibody diluted 1/1000 was used as the secondary.  Cells were counterstained with the nuclear TOPRO-3 stain.

    See Abreview

  • Lane 1: ab13534 + Mouse brain lysate (250µg)
    Lane 2: ab13534 + Mouse brain lysate (250µg, ½ dilution)
    Lane 3: Mouse brain lysate (15µg)
    Lane 4: Rat brain lysate (15µg)

    Ab13534 at 1:1000 immunoprecipitating SOD2 in mouse brain and rat brain lysate. For western blotting, donkey anti- rabbit IgG (HRP) was used as the secondary antibody.

  • All lanes : Anti-SOD2/MnSOD antibody (ab13534) at 1/2000 dilution

    Lane 1 : 40ug Fly Larvae
    Lane 2 : 40ug Fly Pre-Pupae
    Lane 3 : 40ug Fly Pupae
    Lane 4 : 40ug Fly Adult

    Secondary
    HRP conjugated Donkey anti-rabbit IgG
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26.6 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 14 kDa (possible non-specific binding),45 kDa (possible non-specific binding),70 kDa (possible non-specific binding).

    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-SOD2/MnSOD antibody (ab13534)

This product has been referenced in:
  • Jiang YF  et al. Electron tomographic analysis reveals ultrastructural features of mitochondrial cristae architecture which reflect energetic state and aging. Sci Rep 7:45474 (2017). WB . Read more (PubMed: 28358017) »
  • Shanmugam G  et al. Constitutive activation of Nrf2 induces a stable reductive state in the mouse myocardium. Redox Biol 12:937-945 (2017). WB ; Mouse . Read more (PubMed: 28482326) »

See all 27 Publications for this product

Product Wall

Application
Western blot
Sample
Silk worm (Bombyx mori) Tissue lysate - whole (testis lysate (silkworm), BmN4 cell lysate (silkwo)
Gel Running Conditions
Non-reduced Denaturing (12% polyacrylamide)
Loading amount
10 µg
Specification
testis lysate (silkworm), BmN4 cell lysate (silkwo
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

陽水 野島

Verified customer

Submitted Aug 17 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (Ileo)
Specification
Ileo
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Username

Abcam user community

Verified customer

Submitted Apr 12 2013

Thank you very much for your email.

These Abreview codes should be valid for 4 months after the products have shipped, so I've updated these codes so that they expire on January 3, 2013.

If there are any further delays or if the cus...

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Thank you very much for your email. I have created the discount codes below, and included information below for the customer.

DISCOUNT CODES:

***

Expiration date: November 27, 2012

Eachcode will give you 1 free p...

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Thank you for contacting us.

While I feel that such testing would be valuable, unfortunately at this time we do not have a peptide testing discount program. We do have an abreview program in which you might post the results of such tests on ...

Read More

Thank you for contacting Abcam and for sending me these questions.


In regards to your first question, we used an anti-SOD antibody for the western blot image. However the particular product we used is not offered by us for sale. We do o...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M Citrate pH6.0 110oC 2' Milestone RHS-2 microwave
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Oct 06 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Ewing's cell lines)
Loading amount
20 µg
Specification
Ewing's cell lines
Gel Running Conditions
Reduced Denaturing (12 % gel)
Blocking step
LiCOR Odyssey Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 25 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (differentiating PC12 cells)
Specification
differentiating PC12 cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton X100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Anne Chiaramello

Verified customer

Submitted Apr 18 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Primary Hepatocytes)
Loading amount
5 µg
Specification
Primary Hepatocytes
Blocking step
Starting Block (Pierce) as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100%
Username

Abcam user community

Verified customer

Submitted Jan 30 2007

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