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Full length protein corresponding to Rat SOD2/MnSOD.
Our Abpromise guarantee covers the use of ab13534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 2 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|WB||1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 26.6 kDa).|
|IP||Use a concentration of 10 µg/ml.|
Primary antibody was incubated for 16 hours at 4°C, at a 1/2000 dilution.Secondary antibody was a goat anti-rabbit conjugated to Alexa Fluor® 680, used at a 1/5000 dilution.
ab13534 at 1/100 dilution staining Superoxide Dismutase 2 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/50 dilution.
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SOD2/MnSOD with ab13534. Cells were fixed with 2% formaldehyde for 20 minutes at room temperature and incubated with the primary antibody at a dilution of 1/120 for 12 hours at 4°C. Followed by incubation with a R-PE-conjugated goat anti-rabbit IgG at a dilution of 1/200 for 2 hours at room temperature. Counterstained with DAPI (blue) at 1/40000 for 2 hours at room temperature. Magnification: 100x.
Left: DAPI (blue) nuclear stain. Middle: SOD2/MnSOD. Right: Merge.
ab13534 staining SOD2 in Human colon and Horse Ileo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/2000) for 18 hours.
ab13534 staining differentiating (rat) PC12 cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton X100 prior to blocking in 10% serum at RT for 1 hour. The primary antibody was incubated with the sample for 1 hour at 2 µg/ml. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody diluted 1/1000 was used as the secondary. Cells were counterstained with the nuclear TOPRO-3 stain.
Lane 1: ab13534 + Mouse brain lysate (250µg)
Lane 2: ab13534 + Mouse brain lysate (250µg, ½ dilution)
Lane 3: Mouse brain lysate (15µg)
Lane 4: Rat brain lysate (15µg)
Ab13534 at 1:1000 immunoprecipitating SOD2 in mouse brain and rat brain lysate. For western blotting, donkey anti- rabbit IgG (HRP) was used as the secondary antibody.