Validated using a knockout cell line

Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)


  • Product name
    Anti-SOD2/MnSOD antibody [EPR2560Y]
    See all SOD2/MnSOD primary antibodies
  • Description
    Rabbit monoclonal [EPR2560Y] to SOD2/MnSOD
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human SOD2/MnSOD aa 1-100 (N terminal).

  • Positive control
    • WB: Rat brain lysate and SH-SY5Y lysate. IHC-P: Human liver tissue sections.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.


Our Abpromise guarantee covers the use of ab68155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 21906276
WB 1/1000 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
    • Involvement in disease
      Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
    • Sequence similarities
      Belongs to the iron/manganese superoxide dismutase family.
    • Post-translational
      Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Indophenoloxidase B antibody
      • IPO B antibody
      • IPOB antibody
      • Manganese containing superoxide dismutase antibody
      • Manganese SOD antibody
      • Manganese superoxide dismutase antibody
      • Mangano superoxide dismutase antibody
      • Mn SOD antibody
      • Mn superoxide dismutase antibody
      • MNSOD antibody
      • MVCD6 antibody
      • SOD 2 antibody
      • SOD2 antibody
      • SODM_HUMAN antibody
      • Superoxide dismutase [Mn] mitochondrial antibody
      • Superoxide dismutase [Mn], mitochondrial antibody
      • Superoxide dismutase 2 mitochondrial antibody
      see all

    Anti-SOD2/MnSOD antibody [EPR2560Y] images

    • Performed under reducing conditions.

      Predicted band size : 25 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: SOD2 knockout HAP1 cell lysate (20 µg)
      Lane 3: Human heart tissue lysate (20 µg)
      Lane 4: Rat heart tissue lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab68155 observed at 29 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab68155 was shown to specifically react with SOD2 when SOD2 knockout samples were used. Wild-type and SOD2 knockout samples were subjected to SDS-PAGE. ab68155 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/500 dilution

      Lane 1 : Rat brain lysate
      Lane 2 : SH-SY5Y lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 25 kDa
      Observed band size : 25 kDa
    • ab68155 at 1:100 dilution staining Superoxide Dismutase 2 in human liver tissue sections.
    • Immunohistochemical analysis of frozen rat lung tissue taken from rats with monocrotaline-exposure/pneumonectomy, staining SOD2 with ab68155.

    References for Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)

    This product has been referenced in:
    • Cheng XY  et al. AMP-activated protein kinase reduces inflammatory responses and cellular senescence in pulmonary emphysema. Oncotarget 8:22513-22523 (2017). WB ; Mouse . Read more (PubMed: 28186975) »
    • Hao XD  et al. Decreased Integrity, Content, and Increased Transcript Level of Mitochondrial DNA Are Associated with Keratoconus. PLoS One 11:e0165580 (2016). WB ; Human . Read more (PubMed: 27783701) »

    See all 5 Publications for this product

    Product Wall

    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (12% tris-glycine gel)
    Human Tissue lysate - whole (heart)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

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    Submitted Oct 23 2014


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