Overview

  • Product nameSodium butyrate
  • Description
    Histone deacetylase inhibitor
  • Biological descriptionHistone deacetylase inhibitor (IC50 values are 0.3, 0.4 and 0.3 mM for HDAC1, 2 and 7, respectively). Shows no inhibition of HDAC6 and 10. Anticancer activity. Crosses the blood-brain barrier.
  • Purity> 98%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120948 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Sodium butyrate images


    • Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 10 seconds

      HeLa cells were incubated at 37°C for 6h with vehicle control (0 µM) and different concentrations of sodium butyrate (ab120948). Increased expression of histone H3 (acetyl K27) (ab4729) in HeLa cells correlates with an increase in sodium butyrate concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 2.5µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab4927 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    • ab4729 staining Histone H3 (acetyl K27) in HeLa cells. The cells were incubated with 10mM Sodium butyrate (ab120948) for 6 hours (Treated) or solvent-only for control purposes (Non-treated). Cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab4729 at 0.5µg/ml and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with a anti-rabbit AlexaFluor®488 secondary antibody (ab150077) at 2 µg/ml (shown in green) and a goat anti-mouse AlexaFluor®594 (ab150120) at 2 µg/ml (shown in pseudo colour red). Nuclear DNA was labelled in blue with DAPI. Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

    References for Sodium butyrate (ab120948)

    This product has been referenced in:
    • Grayson DR  et al. Is there a future for histone deacetylase inhibitors in the pharmacotherapy of psychiatric disorders? Mol Pharmacol 77:126-35 (2010). Read more (PubMed: 19917878) »
    • Pajak B  et al. Molecular basis of sodium butyrate-dependent proapoptotic activity in cancer cells. Adv Med Sci 52:83-8 (2007). Read more (PubMed: 18217395) »
    • Gurvich N  et al. Histone deacetylase is a target of valproic acid-mediated cellular differentiation. Cancer Res 64:1079-86 (2004). Read more (PubMed: 14871841) »
    • Guardiola AR & Yao TP Molecular cloning and characterization of a novel histone deacetylase HDAC10. J Biol Chem 277:3350-6 (2002). Read more (PubMed: 11726666) »

    See 0 Publications for this product

    Product Wall



    It looks like sodium butyrate can be added to a lysis buffer for histone deactylase inhibition-

    http://www.nature.com/protocolexchange/protocols/500#/reagents

    http://methdb.univ-perp.fr/cgrunau/methods/native_chip_sm.html ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"