Overview

  • Product nameAnti-Sodium / Hydrogen Exchanger 1 antibody
    See all Sodium / Hydrogen Exchanger 1 primary antibodies
  • Description
    Mouse monoclonal to Sodium / Hydrogen Exchanger 1
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: VLRSHGLQLS PTASTIRSSE PPRERSIGDV TTAPPEVTPE SRPVNHSVTD HGMKPRKAFP VLGIDYTHVR TPFEISLWIL LACLMKIGFH VIPTISSIVP , corresponding to amino acids 31-130 of Human Sodium/ Hydrogen Exchanger 1

Properties

Applications

Our Abpromise guarantee covers the use of ab58304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionInvolved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
  • Tissue specificityKidney and intestine.
  • Sequence similaritiesBelongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    O-glycosylated.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • amiloride-sensitive antibody
    • APNH antibody
    • APNH1 antibody
    • FLJ42224 antibody
    • Na Li countertransporter antibody
    • Na(+)/H(+) antiporter antibody
    • Na(+)/H(+) exchanger 1 antibody
    • Na+ H+ antiporter amiloride-sensitive antibody
    • Na+ H+ antiporter antibody
    • Na+ H+ exchanger 1 antibody
    • NHE-1 antibody
    • NHE1 antibody
    • OTTHUMP00000004468 antibody
    • SL9A1_HUMAN antibody
    • SLC9A1 antibody
    • Sodium hydrogen exchanger 1 antibody
    • Sodium/hydrogen exchanger 1 antibody
    • solute carrier family 9 antibody
    • Solute carrier family 9 member 1 antibody
    • Solute carrier family 9 sodium hydrogen exchanger isoform 1 antiporter Na+ H+ amiloride sensitive antibody
    • Solute carrier family 9 subfamily A (NHE1 cation proton antiporter 1) member 1 antibody
    • Solute carrier family 9 subfamily A member 1 antibody
    see all

Anti-Sodium / Hydrogen Exchanger 1 antibody images

  • Sodium / Hydrogen Exchanger 1 antibody (ab58304) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.
  • Western blot against tagged recombinant protein immunogen using ab58304 Sodium / Hydrogen Exchanger 1 antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa
  • Overlay histogram showing HEK293 cells stained with ab58304 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58304, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed HEK293 cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-Sodium / Hydrogen Exchanger 1 antibody (ab58304)

This product has been referenced in:
  • Yang X  et al. Inhibition of Na+/H+ exchanger 1 by cariporide reduces burn-induced intestinal barrier breakdown. Burns 39:1557-64 (2013). IHC-P . Read more (PubMed: 23683660) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"