The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 21705333
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).
Use a concentration of 20 µg/ml.
Use at an assay dependent concentration.
Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
Kidney and intestine.
Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
Phosphorylated upon DNA damage, probably by ATM or ATR. O-glycosylated.
Kao L et al. Severe Neurologic Impairment in Mice with Targeted Disruption of the Electrogenic Sodium Bicarbonate Cotransporter NBCe2 (Slc4a5 Gene). J Biol Chem286:32563-74 (2011).
Read more (PubMed: 21705333) »
Uray KS et al. Sodium hydrogen exchanger as a mediator of hydrostatic edema-induced intestinal contractile dysfunction. Surgery149:114-25 (2011).
Read more (PubMed: 20553904) »