The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 21705333
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).
Use a concentration of 20 µg/ml.
Use at an assay dependent concentration.
FunctionInvolved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
Tissue specificityKidney and intestine.
Sequence similaritiesBelongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. O-glycosylated.
Immunofluorescence of Nhe-1 in Human Brain cells using ab67313 at 20 ug/ml.
References for Anti-Sodium / Hydrogen Exchanger 1 antibody (ab67313)
This product has been referenced in:
Kao L et al. Severe Neurologic Impairment in Mice with Targeted Disruption of the Electrogenic Sodium Bicarbonate Cotransporter NBCe2 (Slc4a5 Gene). J Biol Chem286:32563-74 (2011).
Read more (PubMed: 21705333) »