Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody (ab95299)
Key features and details
- Rabbit polyclonal to Sodium/Hydrogen Exchanger 3/NHE-3
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody
See all Sodium/Hydrogen Exchanger 3/NHE-3 primary antibodies -
Description
Rabbit polyclonal to Sodium/Hydrogen Exchanger 3/NHE-3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide corresponding to Rat Sodium/Hydrogen Exchanger 3/NHE-3 aa 809-831 (C terminal).
Sequence:DSFLQADGPEEQLQPASPESTHM
Database link: NP_036786 -
Positive control
- Rat kidney tissue lysate. IHC-P: rat colon tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 93 kDa).
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IHC-P |
1/25.
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Notes |
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WB
1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 93 kDa). |
IHC-P
1/25. |
Target
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Function
Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. -
Sequence similarities
Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. -
Post-translational
modificationsPhosphorylated by PKA, which inhibits activity. -
Cellular localization
Membrane. In intestinal epithelial cells, localizes to the ileal brush border. - Information by UniProt
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Database links
- Entrez Gene: 24784 Rat
- SwissProt: P26433 Rat
- Unigene: 9706 Rat
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Alternative names
- MGC126718 antibody
- MGC126720 antibody
- Na(+)/H(+) exchanger 3 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded formalin fixed (4%) rat colon tissue labelling Exchanger 3/NHE-3 with ab95299 at 1/25 dilution, followed by secondary antibody Goat Anti-Rabbit IgG (Alexa Fluor 488) (Green). Membrane and secretory staining on rat colon tissue is observed. Counter stained with Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 (Red) F-Actin stain. (C) Exchanger 3/NHE-3 Antibody (D) Composite image.
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Western blot detection of Sodium/Hydrogen Exchanger 3 / NHE-3 in Rat kidney tissues using ab95299 at 1:1000 dilution.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab95299 has been referenced in 5 publications.
- Zarei K et al. Anion Transport Across Human Gallbladder Organoids and Monolayers. Front Physiol 13:882525 (2022). PubMed: 35685290
- Wang JL et al. Expression, Localization, and Effect of High Salt Intake on Electroneutral Na+/HCO3- Cotransporter NBCn2 in Rat Small Intestine: Implication in Intestinal NaCl Absorption. Front Physiol 10:1334 (2019). PubMed: 31736772
- Tingskov SJ et al. Tamoxifen Decreases Lithium-Induced Natriuresis in Rats With Nephrogenic Diabetes Insipidus. Front Physiol 9:903 (2018). PubMed: 30050465
- Wang YY et al. SLC9A3 Protein Is Critical for Acrosomal Formation in Postmeiotic Male Germ Cells. Int J Mol Sci 19:N/A (2017). PubMed: 29286340
- Wang YY et al. Loss of SLC9A3 decreases CFTR protein and causes obstructed azoospermia in mice. PLoS Genet 13:e1006715 (2017). IHC-P ; Mouse . PubMed: 28384194