Anti-Sodium / Hydrogen Exchanger 3 (phospho S552) antibody [14D5] (ab72729)

Overview

  • Product name
    Anti-Sodium / Hydrogen Exchanger 3 (phospho S552) antibody [14D5]
    See all Sodium / Hydrogen Exchanger 3 primary antibodies
  • Description
    Mouse monoclonal [14D5] to Sodium / Hydrogen Exchanger 3 (phospho S552)
  • Tested applications
    Suitable for: WB, IP, ELISA, ICC/IF, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human, Opossum
  • Immunogen

    Synthetic phospho-peptide(rat) made from residues surrounding Serine 552.

  • Positive control
    • human kidney lysate
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: Tris glycine, 150mM Sodium chloride
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    14D5
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 93 kDa).
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF 1/50 - 1/1000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
  • Sequence similarities
    Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
  • Post-translational
    modifications
    Phosphorylated by PKA, which inhibits activity.
  • Cellular localization
    Membrane. In intestinal epithelial cells, localizes to the ileal brush border.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC126718 antibody
    • MGC126720 antibody
    • Na(+)/H(+) exchanger 3 antibody
    • NHE 3 antibody
    • NHE-3 antibody
    • NHE3 antibody
    • SL9A3_HUMAN antibody
    • SLC9A 3 antibody
    • Slc9a3 antibody
    • Sodium/hydrogen exchanger 3 antibody
    • Sodium/hydrogen exchanger, apical epithelial antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), member 3 antibody
    • Solute carrier family 9 member 3 antibody
    see all

Images

  • Anti-Sodium / Hydrogen Exchanger 3 (phospho S552) antibody [14D5] (ab72729) at 5 µg/ml + human kidney lysate

    Predicted band size : 93 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

References

ab72729 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry.

The antibody has been tested in Western Blot using Human, Mouse, and Rat Colon, Small Intestine, and Kidney samples. There were bands expressed around 25kDa and 50kDa, with weaker bands at approx. 80-90 kDa observ...

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Thank you for your enquiry. These antibodies are phosphospecific and will not cross react with the non-phospho antigen. They will only detect the whole protein if it is phosphorylated at the repective serine residues. I hope this information hel...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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