• Product nameAnti-Sodium Potassium ATPase antibody
    See all Sodium Potassium ATPase primary antibodies
  • Description
    Rabbit polyclonal to Sodium Potassium ATPase
  • Specificityab58475 detects endogenous levels of total Sodium Potassium ATPase protein.
  • Tested applicationsSuitable for: IHC-Fr, ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human Sodium Potassium ATPase around the phosphorylation site of serine 16 (A-V-SP-E-H).

  • Positive control
    • Human brain tissue and 293 cell extracts treated with PMA (125ng/ml, 30mins).



Our Abpromise guarantee covers the use of ab58475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: 1/5000.
    IHC-Fr: Use at an assay dependent dilution (PMID 19923323).
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThis is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
    • Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
    • Post-translational
      Phosphorylation on Tyr-10 modulates pumping activity.
    • Cellular localizationCell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATPase Na+/K+ transporting alpha antibody
      • adenosinetriphosphatase antibody
      • AT1A1_HUMAN antibody
      • ATP1A1 antibody
      • ATP1A4 antibody
      • ATP1AL2 antibody
      • ATP1B antibody
      • ATP1B1 antibody
      • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
      • ATPase Na+/K+ transporting alpha 4 polypeptide antibody
      • ATPase Na+/K+ transporting beta 1 polypeptide antibody
      • ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
      • ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
      • Beta 1-subunit of Na(+),K(+)-ATPase antibody
      • Na(+)/K(+) ATPase alpha-1 subunit antibody
      • Na(+)/K(+) ATPase alpha-4 subunit antibody
      • Na+, K+ ATPase alpha subunit antibody
      • Na+/K+ ATPase 1 antibody
      • Na+/K+ ATPase 4 antibody
      • Na+/K+ ATPase, alpha-D polypeptide antibody
      • Na, K-ATPase beta-1 polypeptide antibody
      • Na, K-ATPase, alpha-A catalytic polypeptide antibody
      • Na,K-ATPase catalytic subunit alpha-A protein antibody
      • Na,K-ATPase subunit alpha-C antibody
      • polypeptide-like 2 antibody
      • Sodium pump 1 antibody
      • sodium pump 4 antibody
      • Sodium pump subunit alpha-1 antibody
      • sodium pump subunit alpha-4 antibody
      • sodium pump subunit beta-1 antibody
      • sodium-potassium ATPase catalytic subunit alpha-1 antibody
      • sodium-potassium ATPase catalytic subunit alpha-4 antibody
      • sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
      • sodium-potassium ATPase, alpha 4 polypeptide antibody
      • sodium-potassium-ATPase, alpha 1 polypeptide antibody
      • Sodium/potassium transporting ATPase alpha 1 chain antibody
      • Sodium/potassium transporting ATPase subunit beta 1 antibody
      • sodium/potassium-dependent ATPase beta-1 subunit antibody
      • sodium/potassium-transporting ATPase alpha-4 chain antibody
      • sodium/potassium-transporting ATPase beta-1 chain antibody
      • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
      • sodium/potassium-transporting ATPase subunit alpha-4 antibody
      see all

    Anti-Sodium Potassium ATPase antibody images

    • All lanes : Anti-Sodium Potassium ATPase antibody (ab58475) at 1/500 dilution

      Lane 1 : 293 cell extracts treated with PMA (125ng/ml, 30mins)
      Lane 2 : 293 cell extracts treated with PMA (125ng/ml, 30mins) with immunising peptide

      Predicted band size : 113 kDa
      Observed band size : 113 kDa
    • ab58475 at 1/50 dilution staining Sodium Potassium ATPase in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

    References for Anti-Sodium Potassium ATPase antibody (ab58475)

    This product has been referenced in:
    • Lovisa S  et al. Epithelial-to-mesenchymal transition induces cell cycle arrest and parenchymal damage in renal fibrosis. Nat Med 21:998-1009 (2015). IHC-P ; Mouse . Read more (PubMed: 26236991) »
    • Leung Y  et al. Label Retaining Cells (LRCs) with Myoepithelial Characteristic from the Proximal Acinar Region Define Stem Cells in the Sweat Gland. PLoS One 8:e74174 (2013). Read more (PubMed: 24058524) »

    See all 4 Publications for this product

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