Overview

  • Product nameSodium salicylate
  • Description
    Cell-permeable anti-inflammatory agent
  • Biological descriptionCell-permeable anti-inflammatory agent. Inhibits cyclooxygenases preventing prostaglandin formation. Inhibits components of the MAPK cascade, NF-κB and AP-1. Induces apoptosis through p38 MAPK activation.
  • Purity> 99%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120746 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Sodium salicylate images

    • THP1 cells were incubated at 37°C for 40h with vehicle control (0 µM) and different concentrations of sodium salicylate (ab120746). Decreased expression of scavenging receptor SR-BI (ab52629) in THP1 cells correlates with an increase in sodium salicylate concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab52629 at 1/2000 dilution and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    References for Sodium salicylate (ab120746)

    This product has been referenced in:
    • Amann R & Peskar BA Anti-inflammatory effects of aspirin and sodium salicylate. Eur J Pharmacol 447:1-9 (2002). Read more (PubMed: 12106797) »
    • Schwenger P  et al. Activation of p38 mitogen-activated protein kinase by sodium salicylate leads to inhibition of tumor necrosis factor-induced IkappaB alpha phosphorylation and degradation. Mol Cell Biol 18:78-84 (1998). Read more (PubMed: 9418855) »
    • Mitchell JA  et al. Sodium salicylate inhibits cyclo-oxygenase-2 activity independently of transcription factor (nuclear factor kappaB) activation: role of arachidonic acid. Mol Pharmacol 51:907-12 (1997). Read more (PubMed: 9187256) »
    • Schwenger P  et al. Inhibition of tumor necrosis factor-induced p42/p44 mitogen-activated protein kinase activation by sodium salicylate. J Biol Chem 271:8089-94 (1996). Read more (PubMed: 8626494) »

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"