Overview

  • Product nameAnti-Sohlh2 antibody
    See all Sohlh2 primary antibodies
  • Description
    Rabbit polyclonal to Sohlh2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 45-198 of Human Sohlh2 (AAH25383; UniProt ID: Q9NX45 isoform 2).

  • Positive control
    • NT2D1, IMR32, U-87MG and MCF7 cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab101402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: for isoform 2, 25 kDa.
IHC-P 1/100 - 1/500. Suggested antigen retrieval: use heat mediated 10 mM Citrate buffer or Tris EDTA buffer (pH8.0).

Target

  • FunctionProbable transcription factor, which may be involved in spermatogenesis and oogenesis.
  • Sequence similaritiesContains 1 bHLH (basic helix-loop-helix) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHe81 antibody
    • FLJ20449 antibody
    • FLJ57222 antibody
    • SOHLH2 antibody
    • Solh2 antibody
    • SOLH2_HUMAN antibody
    • SPATA28 antibody
    • Spermatogenesis and oogenesis specific basic helix loop helix 2 antibody
    • Spermatogenesis associated 28 antibody
    • Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 2 antibody
    • TEB1 antibody
    see all

Anti-Sohlh2 antibody images

  • All lanes : Anti-Sohlh2 antibody (ab101402) at 1/1000 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : MCF7 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : for isoform 2, 25 kDa
  • Immunohistochemical analysis of paraffin embedded Ca922 xenograft, using ab101402 at a 1/500 dilution.

References for Anti-Sohlh2 antibody (ab101402)

This product has been referenced in:
  • Zhang H  et al. Sohlh2 inhibits ovarian cancer cell proliferation by upregulation of p21 and downregulation of cyclin D1. Carcinogenesis 35:1863-71 (2014). Read more (PubMed: 24858206) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample Marmoset (common) Tissue sections (Adult testis)
Specification Adult testis
Permeabilization No
Fixative Formaldehyde
Username

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Verified customer

Submitted Nov 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"