The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/20000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
1/50 - 1/100.
May act as the primary docking protein directing membrane turnover and assembly of the transient receptor potential channels TRPC4 and TRPC5. Binds phospholipids such as phosphatidylinositol monophosphates, phosphatidylinositol diphosphates (PIP2s) and phosphatidic acid, but not less polar lipids including phosphatidylcholine, phosphatidylserine, and phosphatidylinositol. The binding to PIP2s is calcium dependent. Might be involved in the plasma membrane localization of CTNNB1.
Broad expression. High expression in thalamus and brain. Significantly expressed in vasculature.
Belongs to the SOLO family. Contains 1 CRAL-TRIO domain. Contains 3 spectrin repeats.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Solo with ab181228 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Western blot - Anti-Solo [EPR13772] antibody (ab181228)
Anti-Solo antibody [EPR13772] (ab181228) at 1/20000 dilution + Human fetal brain lysate at 20 µg
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Solo with ab181228 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.