Anti-soluble IL2 Receptor gamma antibody [38024.11] (ab50258)

Overview

  • Product nameAnti-soluble IL2 Receptor gamma antibody [38024.11]
  • Description
    Mouse monoclonal [38024.11] to soluble IL2 Receptor gamma
  • SpecificityBy ELISA, the antibody does not cross react with recombinant human IL2 R alpha, IL2 R beta, IL1 RI, IL1 RII, IL4 R , IL6 R, IL7 R and IL10 R.
  • Tested applicationsSuitable for: WB, Neutralising, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human IL2 soluble receptor gamma.

  • General notes


    Monoclonal anti-Human IL2 sRg may be used to block the biological activity mediated by IL2 and for neutralization of the biological activity mediated by IL2 Rg and the detection of IL2 Rg.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThe antibody was purified from ascites fluid.
  • Primary antibody notesMonoclonal anti-Human IL2 sRg may be used to block the biological activity mediated by IL2 and for neutralization of the biological activity mediated by IL2 Rg and the detection of IL2 Rg.
  • ClonalityMonoclonal
  • Clone number38024.11
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa.

This working dilution was determined using recombinant human IL2 Rg at 100 ng/lane under non-reducing conditions.

Neutralising Use a concentration of 2 - 6 µg/ml.

This concentration was found to block 50% of the bioactivity due to 10 ng/ml recombinant human IL2 in a 3H-thymidine incorporation assay using 105 cells/ml MO7e cells.

Indirect ELISA Use a concentration of 0.5 - 1 µg/ml.

I-ELISA: Use at a concentration of 0.5 - 1 µg/ml (to a limit of 3 ng/well).

Target

  • RelevanceA common subunit for the receptors for a variety of interleukins, the gamma chain is common to the IL2, IL4, IL7, IL21 and probably also the IL13 receptors. Defects in IL2RG are the cause of X-linked severe combined immunodeficiency. The biological effects of IL2R signals are much more complex than simply mediating T-cell growth. Depending on conditions, IL2R signals may also promote cell survival, effector function, and apoptosis. These sometimes contradictory effects underscore the fact that a diversity of intracellular signalling pathways are potentially activated by IL2R. There are at least 3 components of the IL2 receptor; IL2R alpha, IL2R beta, and IL2R gamma chains.
  • Cellular localizationMembrane; single-pass type I membrane protein
  • Database links
  • Alternative names
    • CD132 antibody
    • Cytokine receptor common gamma chain antibody
    • Gamma-C antibody
    • gammaC antibody
    • IL-2 receptor subunit gamma antibody
    • IL-2RG antibody
    • IL2R gamma chain antibody
    • IL2RG antibody
    • IMD4 antibody
    • Interleukin-2 receptor subunit gamma antibody
    • L-2R subunit gamma antibody
    • p64 antibody
    see all

References for Anti-soluble IL2 Receptor gamma antibody [38024.11] (ab50258)

ab50258 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"