Purification notesThe antibody was purified from ascites fluid.
Primary antibody notesMonoclonal anti-Human IL2 sRg may be used to block the biological activity mediated by IL2 and for neutralization of the biological activity mediated by IL2 Rg and the detection of IL2 Rg.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa.
This working dilution was determined using recombinant human IL2 Rg at 100 ng/lane under non-reducing conditions.
Use a concentration of 2 - 6 µg/ml.
This concentration was found to block 50% of the bioactivity due to 10 ng/ml recombinant human IL2 in a 3H-thymidine incorporation assay using 105 cells/ml MO7e cells.
Use a concentration of 0.5 - 1 µg/ml.
I-ELISA: Use at a concentration of 0.5 - 1 µg/ml (to a limit of 3 ng/well).
RelevanceA common subunit for the receptors for a variety of interleukins, the gamma chain is common to the IL2, IL4, IL7, IL21 and probably also the IL13 receptors. Defects in IL2RG are the cause of X-linked severe combined immunodeficiency. The biological effects of IL2R signals are much more complex than simply mediating T-cell growth. Depending on conditions, IL2R signals may also promote cell survival, effector function, and apoptosis. These sometimes contradictory effects underscore the fact that a diversity of intracellular signalling pathways are potentially activated by IL2R. There are at least 3 components of the IL2 receptor; IL2R alpha, IL2R beta, and IL2R gamma chains.
Cellular localizationMembrane; single-pass type I membrane protein