Anti-Soluble Liver/Pancreas Antigen antibody (ab56033)

Overview

  • Product name
    Anti-Soluble Liver/Pancreas Antigen antibody
  • Description
    Rabbit polyclonal to Soluble Liver/Pancreas Antigen
  • Specificity
    This antibody reacts with Soluble Liver/Pancreas Antigen isoform 2, NP_722547.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Jurkat cell lysate (Western Blot). Human Kidney tissue (IHC-P).

Properties

Applications

Our Abpromise guarantee covers the use of ab56033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • Function
    Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.
  • Tissue specificity
    Primarily expressed in liver, pancreas, kidney and lung. Overexpressed in PHA-stimulated T-cells.
  • Pathway
    Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route): step 2/2.
  • Involvement in disease
    Defects in SEPSECS are the cause of pontocerebellar hypoplasia type 2D (PCH2D) [MIM:613811]. PCH2D is a disorder characterized by postnatal onset of progressive atrophy of the cerebrum and cerebellum, microcephaly, profound mental retardation, spasticity, and variable seizures.
  • Sequence similarities
    Belongs to the SepSecS family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434B1417 antibody
    • Liver pancreas antigen antibody
    • Liver-pancreas antigen antibody
    • LP antibody
    • MGC161491 antibody
    • O phosphoseryl tRNA(Sec) selenium transferase antibody
    • O-phosphoseryl-tRNA(Sec) selenium transferase antibody
    • Sec synthase antibody
    • Selenocysteine synthase antibody
    • Selenocysteinyl tRNA(Sec) synthase antibody
    • Selenocysteinyl-tRNA(Sec) synthase antibody
    • Sep (O phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase antibody
    • Sep tRNA:Sec tRNA synthase antibody
    • Sep-tRNA:Sec-tRNA synthase antibody
    • SepSecS antibody
    • SLA antibody
    • SLA p35 antibody
    • SLA-p35 antibody
    • SLA/LP antibody
    • SLA/LP autoantigen antibody
    • Soluble liver antigen antibody
    • Soluble liver antigen/liver pancreas antigen antibody
    • SPCS_HUMAN antibody
    • tRNA(Ser/Sec) associated antigenic protein antibody
    • tRNA(Ser/Sec)-associated antigenic protein antibody
    • TRNP48 antibody
    • UGA suppressor tRNA associated protein antibody
    • UGA suppressor tRNA-associated protein antibody
    see all

Images

  • Anti-Soluble Liver/Pancreas Antigen antibody (ab56033) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Soluble Liver/Pancreas Antigen with ab56033 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References

ab56033 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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