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Anti-Soluble TNF Receptor II antibody [22221.311] (ab10503)


  • Product nameAnti-Soluble TNF Receptor II antibody [22221.311]
    See all Soluble TNF Receptor II primary antibodies
  • Description
    Mouse monoclonal [22221.311] to Soluble TNF Receptor II
  • SpecificityBy immunoblotting and ELISA, the antibody shows no cross reactivity with recombinant human sTNF-RI.
  • Tested applicationsELISA, WB, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human sTNF-RII, expressed in E. coli.

  • General notesEndotoxin level is < 10 ng per mg antibody as determined by the LAL method.



Our Abpromise guarantee covers the use of ab10503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: Use at a concentration of 0.5 - 1 µg/ml, this concentration is determined to detect recombinant human sTNF RII to a limit of 1.25 ng/well.
    Neut: Use at a concentration of 0.5 - 1 µg/ml, this concentration will block 50% of the bioactivity due to 0.3 µg/ml recombinant human sTNF RII, in the presence of 0.25 ng/ml TNF-alpha in a crystal violet cytolytic assay using mouse L929 cells.
    WB: Use at a concentration of 1 - 2 µg/ml, this concentration is determined using recombinant human sTNF RII at 50 ng/lane under non-reducing conditions.Predicted molecular weight: 75 kDa.

    Anti-Human sTNF RII is tested for its ability to neutralize the biological effect of sTNF-RII but not sTNF RI in a cytolytic assay using mouse L929 cells, in the presence of TNF-alpha. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of the soluble receptor activity on a responsive cell line, when the soluble receptor is present at a concentration just high enough to elicit a maximum response.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
    • Sequence similaritiesContains 4 TNFR-Cys repeats.
    • Post-translational
      Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
      A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD120b antibody
      • p75 antibody
      • p75 TNF receptor antibody
      • p75TNFR antibody
      • p80 TNF alpha receptor antibody
      • p80 TNF-alpha receptor antibody
      • Soluble TNFR1B variant 1 antibody
      • TBP-2 antibody
      • TBPII antibody
      • TNF R II antibody
      • TNF R2 antibody
      • TNF R75 antibody
      • TNF-R2 antibody
      • TNF-RII antibody
      • TNFBR antibody
      • TNFR-II antibody
      • TNFR1B antibody
      • TNFR2 antibody
      • TNFR80 antibody
      • Tnfrsf1b antibody
      • TNR1B_HUMAN antibody
      • Tumor necrosis factor beta receptor antibody
      • Tumor necrosis factor binding protein 2 antibody
      • Tumor necrosis factor receptor 2 antibody
      • Tumor necrosis factor receptor superfamily, member 1B antibody
      • Tumor necrosis factor receptor type II antibody
      • Tumor necrosis factor-binding protein 2 antibody
      see all

    References for Anti-Soluble TNF Receptor II antibody [22221.311] (ab10503)

    This product has been referenced in:
    • Bhattacharyya S  et al. Tumor Necrosis Factor {alpha}-induced Inflammation Is Increased but Apoptosis Is Inhibited by Common Food Additive Carrageenan. J Biol Chem 285:39511-22 (2010). Neutralising ; Human . Read more (PubMed: 20937806) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. For both products, the immunogen is the non-glycosylated, N-terminal methionyl form of the naturally-occurring human soluble type II receptor for TNF, minus 53 amino acids from the proline-rich region just exterior to the...

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