Anti-Soluble TNF Receptor II antibody [22221.311] (ab10503)

Overview

  • Product nameAnti-Soluble TNF Receptor II antibody [22221.311]
    See all Soluble TNF Receptor II primary antibodies
  • Description
    Mouse monoclonal [22221.311] to Soluble TNF Receptor II
  • SpecificityBy immunoblotting and ELISA, the antibody shows no cross reactivity with recombinant human sTNF-RI.
  • Tested applicationsELISA, WB, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human sTNF-RII, expressed in E. coli.

  • General notesEndotoxin level is < 10 ng per mg antibody as determined by the LAL method.

Properties

Applications

Our Abpromise guarantee covers the use of ab10503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
Neutralising
  • Application notesELISA: Use at a concentration of 0.5 - 1 µg/ml, this concentration is determined to detect recombinant human sTNF RII to a limit of 1.25 ng/well.
    Neut: Use at a concentration of 0.5 - 1 µg/ml, this concentration will block 50% of the bioactivity due to 0.3 µg/ml recombinant human sTNF RII, in the presence of 0.25 ng/ml TNF-alpha in a crystal violet cytolytic assay using mouse L929 cells.
    WB: Use at a concentration of 1 - 2 µg/ml, this concentration is determined using recombinant human sTNF RII at 50 ng/lane under non-reducing conditions.Predicted molecular weight: 75 kDa.

    Anti-Human sTNF RII is tested for its ability to neutralize the biological effect of sTNF-RII but not sTNF RI in a cytolytic assay using mouse L929 cells, in the presence of TNF-alpha. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of the soluble receptor activity on a responsive cell line, when the soluble receptor is present at a concentration just high enough to elicit a maximum response.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
    • Sequence similaritiesContains 4 TNFR-Cys repeats.
    • Post-translational
      modifications
      Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
      A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD120b antibody
      • Etanercept antibody
      • p75 antibody
      • p75 TNF receptor antibody
      • p75TNFR antibody
      • p80 TNF alpha receptor antibody
      • p80 TNF-alpha receptor antibody
      • Soluble TNFR1B variant 1 antibody
      • TBP-2 antibody
      • TBPII antibody
      • TNF R II antibody
      • TNF R2 antibody
      • TNF R2 antibody
      • TNF R75 antibody
      • TNF-R2 antibody
      • TNF-RII antibody
      • TNFBR antibody
      • TNFR-II antibody
      • TNFR1B antibody
      • TNFR2 antibody
      • TNFR80 antibody
      • Tnfrsf1b antibody
      • TNR1B_HUMAN antibody
      • Tumor necrosis factor beta receptor antibody
      • Tumor necrosis factor binding protein 2 antibody
      • Tumor necrosis factor receptor 2 antibody
      • Tumor necrosis factor receptor superfamily member 1B antibody
      • Tumor necrosis factor receptor superfamily, member 1B antibody
      • Tumor necrosis factor receptor type II antibody
      • Tumor necrosis factor-binding protein 2 antibody
      see all

    References for Anti-Soluble TNF Receptor II antibody [22221.311] (ab10503)

    This product has been referenced in:
    • Bhattacharyya S  et al. Tumor Necrosis Factor {alpha}-induced Inflammation Is Increased but Apoptosis Is Inhibited by Common Food Additive Carrageenan. J Biol Chem 285:39511-22 (2010). Neutralising ; Human . Read more (PubMed: 20937806) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. For both products, the immunogen is the non-glycosylated, N-terminal methionyl form of the naturally-occurring human soluble type II receptor for TNF, minus 53 amino acids from the proline-rich region just exterior to the...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"