The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
RelevanceSolute carrier family 22 member 18 may act as a transporter of organic cations based on a proton efflux antiport mechanism. It may also play a role in the transport of chloroquine and quinidine-related compounds in kidney.
Cellular localizationApical cell membrane; Multi-pass membrane protein (Potential). Note: Localized at the apical membrane surface of renal proximal tubules.