Anti-Somatostatin antibody [8.F.258] (ab30788)

Overview

  • Product nameAnti-Somatostatin antibody [8.F.258]
    See all Somatostatin primary antibodies
  • Description
    Rat monoclonal [8.F.258] to Somatostatin
  • SpecificityThis antibody recognizes somatostatin. It partially cross reacts with somatostatin fragments, but does not cross react with enkephalins, other endorphins, Substance P or CGRP.
  • Tested applicationsSuitable for: ELISA, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein corresponding to Human Somatostatin. Somatostatin conjugated to Thyroglobulin
    Database link: P61278

Properties

Applications

Our Abpromise guarantee covers the use of ab30788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr 1/25 - 1/100.
IHC-P 1/25 - 1/100. Antigen retrieval is recommended. Suitable for IHC(PFA fixed) PMID 21343251.
ICC/IF Use at an assay dependent concentration. PubMed: 23639079

Target

Anti-Somatostatin antibody [8.F.258] images

  • ab30788 staining Somatostatin in mouse embryonic heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 5% serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/100) for 14 hours at 4°C. An Alexa Fluor®-conjugated Donkey anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

References for Anti-Somatostatin antibody [8.F.258] (ab30788)

This product has been referenced in:
  • Higurashi N  et al. A human Dravet syndrome model from patient induced pluripotent stem cells. Mol Brain 6:19 (2013). ICC/IF ; Human . Read more (PubMed: 23639079) »
  • Cai T  et al. Expression of Insulinoma-Associated 2 (INSM2) in Pancreatic Islet Cells Is Regulated by the Transcription Factors Ngn3 and NeuroD1. Endocrinology 152:1961-9 (2011). IHC (PFA fixed) ; Human . Read more (PubMed: 21343251) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Embryonic Heart)
Permeabilization Yes - Tween-20
Specification Embryonic Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Oct 31 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 30 µg
Specification Heart
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Oct 20 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas, langerhans islet, insulin + somatostatin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Permeabilization Yes - Tween-20
Specification pancreas, langerhans islet, insulin + somatostatin
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde
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Submitted Jun 23 2016




I am very pleased to hear you would like to accept our offer and test ab14993, ab30788 ,ab64053 and ab115489 in sELISA. These codes will give you: 1 freeprimary antibody per codebefore the expiration date. To redeem this offer, please s...

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Thank you very much for your interest in PEDF and Somatostatin antibodies.

To our knowledge,none of our antibodies have been tested in sELISA. However, I have picked out four antibodies, which I found most suitable for this purpose:
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Thank you for contacting us. The immunogen sequence of ab53165 is from xxxxxx of human somatostatin. We haven’t analyzed the Kd of this product. This antibody is guaranteed inICC/IF,WB,ELISA,IHC-P on mouse and human tissues. Ab...

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Thank you for contacting us. The immunogen sequence of ab11770 includes amino acids XXX of Human Somatostatin. We haven’t analyzed the Kd of this product. According to our results, this antibody works well in ELISA and WB for purifi...

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Thank you for your enquiry. The antibody does cross-react with Somatostatin 28. The manufacturing lab confirmed that the statement on the datasheet "Partially crossreacts with somatostatin fragments" means that there is a signal, but not as ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"