Mouse, Human Predicted to work with:
Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to Human Somatostatin aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin. Database link: P61278 (Peptide available as ab131563)
This antibody gave a positive signal in Human normal pancreas formalin-fixed, paraffin-embedded tissue section within Immunohistochemistry as well as a positive signal in Mouse Placenta tissue lysate and JEG3 whole cell lysate within Western Blot.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
IHC image of Somatostatin staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108456, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-Somatostatin antibody (ab108456)
All lanes : Anti-Somatostatin antibody (ab108456) at 1 µg/ml
Lane 1 : Mouse Placenta Tissue Lysate Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Alfonsi G & Crippa M Updates on the Methodological Approaches for Carrying Out an In-Depth Study of the Cardiac Conduction System and the Autonomic Nervous System of Victims of Sudden Unexplained Fetal and Infant Death. Front Med (Lausanne)3:54 (2016).
Read more (PubMed: 27917382) »