Anti-Somatostatin antibody [M09204] (ab30788)

Overview

  • Product name
    Anti-Somatostatin antibody [M09204]
    See all Somatostatin primary antibodies
  • Description
    Rat monoclonal [M09204] to Somatostatin
  • Specificity
    This antibody recognizes somatostatin. It partially cross reacts with somatostatin fragments, but does not cross react with enkephalins, other endorphins, Substance P or CGRP. The clone number has been updated from (8.F.258) to (M09204) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: ELISA, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein corresponding to Human Somatostatin. Somatostatin conjugated to Thyroglobulin
    Database link: P61278

Properties

Applications

Our Abpromise guarantee covers the use of ab30788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr 1/25 - 1/100.
IHC-P 1/25 - 1/100. Antigen retrieval is recommended. Suitable for IHC(PFA fixed) PMID 21343251.
ICC/IF Use at an assay dependent concentration. PubMed: 23639079

Target

Images

  • ab30788 staining Somatostatin in mouse embryonic heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 5% serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/100) for 14 hours at 4°C. An Alexa Fluor®-conjugated Donkey anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Gross S  et al. The novel enterochromaffin marker Lmx1a regulates serotonin biosynthesis in enteroendocrine cell lineages downstream of Nkx2.2. Development 143:2616-28 (2016). IHC-P, IHC (PFA fixed) ; Mouse . Read more (PubMed: 27287799) »
  • Szabo NE  et al. Hoxb8 intersection defines a role for Lmx1b in excitatory dorsal horn neuron development, spinofugal connectivity, and nociception. J Neurosci 35:5233-46 (2015). IHC-FoFr ; Mouse . Read more (PubMed: 25834049) »

See all 8 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6,0
Permeabilization
No
Specification
pancreas
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

Submitted Nov 30 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate pH6
Permeabilization
No
Specification
Pancreas
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Jul 14 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic Heart)
Permeabilization
Yes - Tween-20
Specification
Embryonic Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

Submitted Oct 31 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Oct 20 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas, langerhans islet, insulin + somatostatin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Permeabilization
Yes - Tween-20
Specification
pancreas, langerhans islet, insulin + somatostatin
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

Submitted Jun 23 2016




I am very pleased to hear you would like to accept our offer and test ab14993, ab30788 ,ab64053 and ab115489 in sELISA. These codes will give you: 1 freeprimary antibody per codebefore the expiration date. To redeem this offer, please s...

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Thank you very much for your interest in PEDF and Somatostatin antibodies.

To our knowledge,none of our antibodies have been tested in sELISA. However, I have picked out four antibodies, which I found most suitable for this purpose:
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Thank you for contacting us. The immunogen sequence of ab53165 is from xxxxxx of human somatostatin. We haven’t analyzed the Kd of this product. This antibody is guaranteed inICC/IF,WB,ELISA,IHC-P on mouse and human tissues. Ab...

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Thank you for contacting us. The immunogen sequence of ab11770 includes amino acids XXX of Human Somatostatin. We haven’t analyzed the Kd of this product. According to our results, this antibody works well in ELISA and WB for purifi...

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Thank you for your enquiry. The antibody does cross-react with Somatostatin 28. The manufacturing lab confirmed that the statement on the datasheet "Partially crossreacts with somatostatin fragments" means that there is a signal, but not as ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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